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Literature summary extracted from

  • Yousefi-Nejad, M.; Hosseinkhani, S.; Khajeh, K.; Ranjbar, B.
    Expression, purification and immobilization of firefly luciferase on alkyl-substituted Sepharose 4B (2007), Enzyme Microb. Technol., 40, 740-746.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expressed in Escherichia coli BL21 cells Photinus pyralis

General Stability

EC Number General Stability Organism
1.13.12.7 immobilization on hexyl-Sepharose reduces the specific activity to 35%, immobilization on octyl-Sepharose reduces the specific activity to 26% Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.004
-
 ATP free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol Photinus pyralis
1.13.12.7 0.008
-
 Luciferin free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis Q27758
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 Ni-NTA agarose column chromatography Photinus pyralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.7 additional information
-
 enzyme from crude extract shows a specific activity of 6100000 relative light units per mg, purified enzyme shows a specific activity of 104000000 relative light units per mg Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 8-anilino-1-naphthalene-sulfonate + ?
-
 Photinus pyralis ?
-
 ?
1.13.12.7 luciferin + O2 + ATP
-
 Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis
1.13.12.7 Luc
-
 Photinus pyralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 25
-
-
 Photinus pyralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.7 40
-
 immobilized enzyme forms are stable for 1 min at 40°C and then lose their activities Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
-
 Photinus pyralis