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Literature summary extracted from

  • Blazquez, B.; Carmona, M.; Garcia, J.L.; Diaz, E.
    Identification and analysis of a glutaryl-CoA dehydrogenase-encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB (2008), Environ. Microbiol., 10, 474-482.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.6 expressed in Escherichia coli D5alpha and MC4100 cells Azoarcus sp.
1.3.8.6 expressed in the Azoarcus sp. CIBdgcdR mutant strain Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.6 additional information the CIBdgcdR mutant strain is unable to grow in pimelate, glutarate or benzoate as sole carbon sources Azoarcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.6 45000
-
 SDS-PAGE Azoarcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.6 Azoarcus sp. B0EVL5
-
-
1.3.8.6 Pseudomonas putida
-
-
-
1.3.8.6 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.6
-
 Pseudomonas putida
1.3.8.6
-
 Azoarcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.6 culture condition:benzoate-grown cell
-
 Pseudomonas putida
-
1.3.8.6 culture condition:benzoate-grown cell
-
 Azoarcus sp.
-
1.3.8.6 culture condition:pimelate-grown cell
-
 Pseudomonas putida
-
1.3.8.6 culture condition:pimelate-grown cell
-
 Azoarcus sp.
-
1.3.8.6 culture condition:pyruvate-grown cell
-
 Pseudomonas putida
-
1.3.8.6 culture condition:pyruvate-grown cell
-
 Azoarcus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.6 0.028
-
 in cells grown in 0.2% (w/v) pyruvate Azoarcus sp.
1.3.8.6 0.169
-
 in cells grown in 3 mM pimelate Azoarcus sp.
1.3.8.6 0.19
-
 in cells grown in 0.2% (w/v) pyruvate and 3 mM pimelate Azoarcus sp.
1.3.8.6 0.216
-
 in cells grown in 3 mM benzoate Azoarcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.6 glutaryl-CoA + ferricenium hexafluorophosphate
-
 Pseudomonas putida crotonyl-CoA + CO2 + ferrocenium hexafluorophosphate
-
 ?
1.3.8.6 glutaryl-CoA + ferricenium hexafluorophosphate
-
 Azoarcus sp. crotonyl-CoA + CO2 + ferrocenium hexafluorophosphate
-
 ?
1.3.8.6 glutaryl-CoA + ferricenium hexafluorophosphate
-
 Pseudomonas putida KT 2240 crotonyl-CoA + CO2 + ferrocenium hexafluorophosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.8.6 GCDH
-
 Pseudomonas putida
1.3.8.6 GCDH
-
 Azoarcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.6 FAD
-
 Pseudomonas putida
1.3.8.6 FAD
-
 Azoarcus sp.