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Literature summary extracted from

  • Perez-Reinado, E.; Roldan, M.D.; Castillo, F.; Moreno-Vivian, C.
    The NprA nitroreductase required for 2,4-dinitrophenol reduction in Rhodobacter capsulatus is a dihydropteridine reductase (2008), Environ. Microbiol., 10, 3174-3183.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.34 expressed as His-tag fusion protein in Escherichia coli Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 25000
-
 x * 25000, SDS-PAGE, weak band at 50000 Da which possibly corresponds to the dimeric form of the enzyme, calculated mass of the His-tagged protein is 24200 Da Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.34 additional information Rhodobacter capsulatus in vivo role as dihydropteridine reductase involved in aromatic amino acids metabolism suggested ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34 recombinant protein using His-tag Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 6,7-dimethyl-7,8-dihydropterin + NADPH + H+ as the quinonoid forms of pterines are very unstable, the reaction is carried out with a dimethyl analogue in the presence of H2O2 and radish peroxidase to allow the continuous formation of 6,7-dimethyl-7,8-dihydropterine, activity is 25fold higher than the nitroreductase activity 2,4-dinitrophenol Rhodobacter capsulatus 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+
-
 ?
1.5.1.34 additional information in vivo role as dihydropteridine reductase involved in aromatic amino acids metabolism suggested Rhodobacter capsulatus ?
-
 ?
1.5.1.34 additional information enzyme shows nitroreductase activity with several different substrates such as 2,4-dinitrophenol, 2,4-dinitrobenzoate and nitrofurazone Rhodobacter capsulatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.34 More x * 25000, SDS-PAGE, weak band at 50000 Da which possibly corresponds to the dimeric form of the enzyme, calculated mass of the His-tagged protein is 24200 Da Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.5.1.34 NprA nitroreductase enzyme shows nitroreductase activity with a wide range of nitroaromatic compounds, but highest activity is detected with the quinonoid form of 6,7-dimethyl-7,8-dihydropterine as substrate, in vivo role as dihydropteridine reductase involved in aromatic amino acids metabolism suggested Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.34 30
-
-
 Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.34 6.5
-
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 FMN one molecule bound per enzyme Rhodobacter capsulatus
1.5.1.34 NADPH
-
 Rhodobacter capsulatus