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Literature summary extracted from

  • Camberg, J.L.; Johnson, T.L.; Patrick, M.; Abendroth, J.; Hol, W.G.; Sandkvist, M.
    Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids (2007), EMBO J., 26, 19-27.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.4.2.8 cardiolipin via the EpsE/cyto-EpsL complex Vibrio cholerae serotype O1
7.4.2.8 phosphatidylglycerol via the EpsE/cyto-EpsL complex Vibrio cholerae serotype O1
7.6.2.1 acidic phospholipids acidic phospholipids, specifically cardiolipin, bind the co-purified EpsE/cyto-EpsL complex and stimulate its ATPase activity 30-130fold, whereas the activity of EpsE alone is unaffected. Removal of the last 11 residues, residues 243-253, from cyto-EpsL prevented cardiolipin binding as well as stimulation of the ATPase activity of EpsE Vibrio cholerae serotype O1
7.6.2.1 cardiolipin binds the co-purified EpsE/cyto-EpsL complex and stimulates its ATPase activity 30-130fold, whereas the activity of EpsE alone is unaffected. Removal of the last 11 residues, residues 243-253, from cyto-EpsL prevents cardiolipin binding as well as stimulation of the ATPase activity of EpsE Vibrio cholerae serotype O1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.8 plasmids pEpsE/EpsL(1-253)His6 and pEpsE/EpsL(1-242)His6 using pET21d+ are constructed, for expression of monomeric EpsE the vector GST-EpsE is constructed Vibrio cholerae serotype O1

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.8 K270A EpsE mutant Vibrio cholerae serotype O1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.1 cytosol EpsE is a cytoplasmic component of the type II secretion system Vibrio cholerae serotype O1 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.1 Mg2+
-
 Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.8 ATP + H2O Vibrio cholerae serotype O1
-
 ADP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.8 Vibrio cholerae serotype O1
-
-
-
7.6.2.1 Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.8 EpsE is copurified with the cytoplasmic domain of EpsL to test whether the ATPase activity of EpsE can be modulated by EpsL, complexes are purified using metal affinity chromatography and gel filtration, monomeric EpsE is obtained following thrombin cleavage of a GST-EpsE fusion protein Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.8 ATP + H2O
-
 Vibrio cholerae serotype O1 ADP + phosphate
-
 ir
7.6.2.1 ATP + H2O + cholera toxin/in through ATP hydrolysis and an interaction with the cytoplasmic membrane protein EpsL, EpsE supports secretion of cholera toxin across the outer membrane Vibrio cholerae serotype O1 ADP + phosphate + cholera toxin/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.8 EpsE cytoplasmic component of the type II secretion system Vibrio cholerae serotype O1
7.4.2.8 protein-secreting ATPase
-
 Vibrio cholerae serotype O1
7.6.2.1 EpsE
-
 Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.4.2.8 37
-
 ATPase activity assay Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4.2.8 8.5
-
 ATPase activity assay Vibrio cholerae serotype O1