EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.3.10 | biotechnology | studies on biosensors and biofuel cell anodes | Trametes ochracea |
1.1.99.29 | biotechnology | PDH could be a very interesting alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied | Agaricus xanthodermus |
1.1.99.29 | biotechnology | PDH is an alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied | Leucoagaricus meleagris |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.10 | expressed in Escherichia coli BL21-DE3, recombinant protein, plasmids pHL2e and pCL22 encoding the E542K mutant used | Trametes ochracea |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.10 | E542K | inserted into plasmid pCL22 | Trametes ochracea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.10 | additional information | - |
additional information | D-mannose, glucosamine, beta-lactose, sucrose and D-maltose lack response signal, higher maltooligosaccharides good substrates for pyranose 2-oxidase-based biosensor | Trametes ochracea | |
1.1.3.10 | 0.32 | - |
D-glucose | wild-type protein, 0.1 M ethanolamine buffer at pH 10.5 | Trametes ochracea | |
1.1.3.10 | 0.35 | - |
D-glucose | recombinant protein with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 | Trametes ochracea | |
1.1.3.10 | 0.71 | - |
D-cellobiose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 1.51 | - |
D-glucose | E542K with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 | Trametes ochracea | |
1.1.3.10 | 1.55 | - |
L-arabinose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 2.72 | - |
D-glucose | recombinant protein, 0.1 M PBS buffer at pH 8 | Trametes ochracea | |
1.1.3.10 | 6.02 | - |
D-maltoheptaose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 6.14 | - |
D-maltopentaose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 6.21 | - |
D-xylose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 6.25 | - |
D-fucose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 6.61 | - |
D-maltotriose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 7.51 | - |
D-trehalose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 10.32 | - |
2-deoxy-D-galactose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 11.81 | - |
D-galactose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.3.10 | 11.85 | - |
2-deoxy-D-glucose | 100 mM ethanolamine buffer, pH 10.5 | Trametes ochracea | |
1.1.99.29 | 0.05 | - |
D-trehalose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.34 | - |
D-maltose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.35 | - |
sucrose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.44 | - |
D-glucose | at 0.1 M ethanolamine buffer at pH 9.4 | Agaricus xanthodermus | |
1.1.99.29 | 0.51 | - |
D-maltoheptaose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.62 | - |
D-mannose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.64 | - |
D-maltotriose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.64 | - |
D-maltopentaose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.65 | - |
D-glucose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.65 | - |
2-deoxy-D-glucose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 0.74 | - |
D-glucosamine | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 1.05 | - |
D-cellobiose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 5.84 | - |
D-xylose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 7.16 | - |
D-galactose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 8.63 | - |
L-arabinose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 10.84 | - |
beta-lactose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 12.14 | - |
2-deoxy-D-galactose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris | |
1.1.99.29 | 12.31 | - |
L-fucose | 0.1 M ethanolamine buffer at pH 9.4 | Leucoagaricus meleagris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.29 | extracellular | - |
Leucoagaricus meleagris | - |
- |
1.1.99.29 | extracellular | - |
Agaricus xanthodermus | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.10 | Trametes ochracea | Q7ZA32 | wild-type, recombinant protein and commercial preparation | - |
1.1.99.29 | Agaricus xanthodermus | Q3L1D2 | - |
- |
1.1.99.29 | Leucoagaricus meleagris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.10 | wild-type and recombinant protein, gel purification | Trametes ochracea |
1.1.99.29 | using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography | Leucoagaricus meleagris |
1.1.99.29 | using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography | Agaricus xanthodermus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | additional information | - |
pyranose 2-oxidase-based biosensor system, electrical wiring of different types of pyranose oxidase with an osmium redox polymer on graphite electrodes, optimization studies using glucose as substrate, biosensors with best characteristics in terms of linear range, detection limit and sensitivity used for analyzing selectivity for different sugar substrates | Trametes ochracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.10 | 2-deoxy-D-galactose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | 2-deoxy-D-glucose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-cellobiose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-fucose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-galactose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-glucose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
1.1.3.10 | D-maltoheptaose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-maltopentaose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-maltotriose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-trehalose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | D-xylose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.3.10 | L-arabinose + O2 | selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed | Trametes ochracea | ? | - |
? | |
1.1.99.29 | 2-deoxy-D-galactose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | 2-deoxy-D-glucose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | beta-lactose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-cellobiose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-galactose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-glucosamine + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-glucose + acceptor | - |
Leucoagaricus meleagris | 3-dehydro-D-glucose + reduced acceptor | - |
? | |
1.1.99.29 | D-glucose + acceptor | - |
Agaricus xanthodermus | 3-dehydro-D-glucose + reduced acceptor | - |
? | |
1.1.99.29 | D-maltoheptaose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-maltopentaose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-maltose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-maltotriose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-mannose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-trehalose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-xylose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | L-arabinose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | L-fucose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | sucrose + acceptor | - |
Leucoagaricus meleagris | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.29 | PDH AM | - |
Leucoagaricus meleagris |
1.1.99.29 | PDH AX | - |
Agaricus xanthodermus |
1.1.99.29 | pyranose dehydrogenase | - |
Leucoagaricus meleagris |
1.1.99.29 | pyranose dehydrogenase | - |
Agaricus xanthodermus |
1.1.99.29 | pyranose:acceptor oxidoreductase | - |
Leucoagaricus meleagris |
1.1.99.29 | pyranose:acceptor oxidoreductase | - |
Agaricus xanthodermus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 4 | - |
under dry and wet storage conditions stable at 4°C, under dry storage conditions retainment of 100% of original activity after 2 days and of about 90% after one week, storage in working buffer solutions decreases activity by about 10% after one day and by more than 50% after one week | Trametes ochracea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 10.5 | - |
activity assay at | Trametes ochracea |
1.1.99.29 | 9 | - |
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM D-glucose, flow rate 1.0 mL/min | Leucoagaricus meleagris |
1.1.99.29 | 9 | - |
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min | Agaricus xanthodermus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 8 | 11 | optimal buffer combination for the pyranose 2-oxidase systems determined | Trametes ochracea |
1.1.99.29 | 7 | 8 | in PBS, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min | Leucoagaricus meleagris |
1.1.99.29 | 7 | 10.5 | depending on the buffer, highest biosensor response in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min | Leucoagaricus meleagris |
1.1.99.29 | 8 | 10 | in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min | Agaricus xanthodermus |
1.1.99.29 | 8 | 9 | in Tris, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min | Leucoagaricus meleagris |