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Literature summary extracted from

  • Tasca, F.; Timur, S.; Ludwig, R.; Haltrich, D.; Volc, J.; Antiochia, R.; Gorton, L.
    Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes (2007), Electroanalysis, 19, 294-302.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.3.10 biotechnology studies on biosensors and biofuel cell anodes Trametes ochracea
1.1.99.29 biotechnology PDH could be a very interesting alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied Agaricus xanthodermus
1.1.99.29 biotechnology PDH is an alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied Leucoagaricus meleagris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expressed in Escherichia coli BL21-DE3, recombinant protein, plasmids pHL2e and pCL22 encoding the E542K mutant used Trametes ochracea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 E542K inserted into plasmid pCL22 Trametes ochracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 additional information
-
additional information D-mannose, glucosamine, beta-lactose, sucrose and D-maltose lack response signal, higher maltooligosaccharides good substrates for pyranose 2-oxidase-based biosensor Trametes ochracea
1.1.3.10 0.32
-
D-glucose wild-type protein, 0.1 M ethanolamine buffer at pH 10.5 Trametes ochracea
1.1.3.10 0.35
-
D-glucose recombinant protein with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 Trametes ochracea
1.1.3.10 0.71
-
D-cellobiose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 1.51
-
D-glucose E542K with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 Trametes ochracea
1.1.3.10 1.55
-
L-arabinose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 2.72
-
D-glucose recombinant protein, 0.1 M PBS buffer at pH 8 Trametes ochracea
1.1.3.10 6.02
-
D-maltoheptaose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 6.14
-
D-maltopentaose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 6.21
-
D-xylose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 6.25
-
D-fucose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 6.61
-
D-maltotriose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 7.51
-
D-trehalose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 10.32
-
2-deoxy-D-galactose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 11.81
-
D-galactose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.3.10 11.85
-
2-deoxy-D-glucose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.1.99.29 0.05
-
D-trehalose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.34
-
D-maltose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.35
-
sucrose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.44
-
D-glucose at 0.1 M ethanolamine buffer at pH 9.4 Agaricus xanthodermus
1.1.99.29 0.51
-
D-maltoheptaose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.62
-
D-mannose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.64
-
D-maltotriose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.64
-
D-maltopentaose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.65
-
D-glucose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.65
-
2-deoxy-D-glucose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 0.74
-
D-glucosamine 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 1.05
-
D-cellobiose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 5.84
-
D-xylose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 7.16
-
D-galactose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 8.63
-
L-arabinose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 10.84
-
beta-lactose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 12.14
-
2-deoxy-D-galactose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.1.99.29 12.31
-
L-fucose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.29 extracellular
-
Leucoagaricus meleagris
-
-
1.1.99.29 extracellular
-
Agaricus xanthodermus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea Q7ZA32 wild-type, recombinant protein and commercial preparation
-
1.1.99.29 Agaricus xanthodermus Q3L1D2
-
-
1.1.99.29 Leucoagaricus meleagris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 wild-type and recombinant protein, gel purification Trametes ochracea
1.1.99.29 using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography Leucoagaricus meleagris
1.1.99.29 using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography Agaricus xanthodermus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 additional information
-
pyranose 2-oxidase-based biosensor system, electrical wiring of different types of pyranose oxidase with an osmium redox polymer on graphite electrodes, optimization studies using glucose as substrate, biosensors with best characteristics in terms of linear range, detection limit and sensitivity used for analyzing selectivity for different sugar substrates Trametes ochracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 2-deoxy-D-galactose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 2-deoxy-D-glucose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-cellobiose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-fucose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-galactose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-glucose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea 2-dehydro-D-glucose + H2O2
-
?
1.1.3.10 D-maltoheptaose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-maltopentaose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-maltotriose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-trehalose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 D-xylose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.3.10 L-arabinose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
?
1.1.99.29 2-deoxy-D-galactose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 2-deoxy-D-glucose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 beta-lactose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-cellobiose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-galactose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-glucosamine + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-glucose + acceptor
-
Leucoagaricus meleagris 3-dehydro-D-glucose + reduced acceptor
-
?
1.1.99.29 D-glucose + acceptor
-
Agaricus xanthodermus 3-dehydro-D-glucose + reduced acceptor
-
?
1.1.99.29 D-maltoheptaose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-maltopentaose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-maltose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-maltotriose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-mannose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-trehalose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 D-xylose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 L-arabinose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 L-fucose + acceptor
-
Leucoagaricus meleagris ?
-
?
1.1.99.29 sucrose + acceptor
-
Leucoagaricus meleagris ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH AM
-
Leucoagaricus meleagris
1.1.99.29 PDH AX
-
Agaricus xanthodermus
1.1.99.29 pyranose dehydrogenase
-
Leucoagaricus meleagris
1.1.99.29 pyranose dehydrogenase
-
Agaricus xanthodermus
1.1.99.29 pyranose:acceptor oxidoreductase
-
Leucoagaricus meleagris
1.1.99.29 pyranose:acceptor oxidoreductase
-
Agaricus xanthodermus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 4
-
under dry and wet storage conditions stable at 4°C, under dry storage conditions retainment of 100% of original activity after 2 days and of about 90% after one week, storage in working buffer solutions decreases activity by about 10% after one day and by more than 50% after one week Trametes ochracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 10.5
-
activity assay at Trametes ochracea
1.1.99.29 9
-
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM D-glucose, flow rate 1.0 mL/min Leucoagaricus meleagris
1.1.99.29 9
-
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min Agaricus xanthodermus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.10 8 11 optimal buffer combination for the pyranose 2-oxidase systems determined Trametes ochracea
1.1.99.29 7 8 in PBS, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min Leucoagaricus meleagris
1.1.99.29 7 10.5 depending on the buffer, highest biosensor response in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min Leucoagaricus meleagris
1.1.99.29 8 10 in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min Agaricus xanthodermus
1.1.99.29 8 9 in Tris, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min Leucoagaricus meleagris