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Literature summary extracted from
- Comparison of the 17 alpha-hydroxylase/C17,20-lyase activities of porcine, guinea pig and bovine P450c17 using purified recombinant fusion proteins containing P450c17 linked to NADPH-P450 reductase (2007), Drug Metab. Rev., 39, 289-307.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | additional information | cytochrome B5 does not affect the 17alpha-hydroxylase activity | Cavia porcellus | |
| 1.14.14.19 | additional information | cytochrome B5 does not affect the 17alpha-hydroxylase activity | Sus scrofa | |
| 1.14.14.19 | additional information | cytochrome B5 does not affect the 17alpha-hydroxylase activity | Bos taurus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.19 | the P450c17 domain of the coding sequence is connected to a truncated form of rat NADPH-P450 reductase by a linker sequence encoding Ser-Thr, functional expression of fusion proteins in Escherichia coli | Cavia porcellus |
| 1.14.14.19 | the P450c17 domain of the coding sequence is connected to a truncated form of rat NADPH-P450 reductase by a linker sequence encoding Ser-Thr, functional expression of fusion proteins in Escherichia coli | Sus scrofa |
| 1.14.14.19 | the P450c17 domain of the coding sequence is connected to a truncated form of rat NADPH-P450 reductase by a linker sequence encoding Ser-Thr, functional expression of fusion proteins in Escherichia coli | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.19 | additional information | Cavia porcellus | the enzyme catalyzes the key branch point in the metabolic conversion of cholesterol either to androgens and estrogens, or to glucocorticoids | ? | - |
? |  |
| 1.14.14.19 | additional information | Sus scrofa | the enzyme catalyzes the key branch point in the metabolic conversion of cholesterol either to androgens and estrogens, or to glucocorticoids | ? | - |
? | |
| 1.14.14.19 | additional information | Bos taurus | the enzyme catalyzes the key branch point in the metabolic conversion of cholesterol either to androgens and estrogens, or to glucocorticoids | ? | - |
? | |
| 1.14.14.19 | pregnenolone + AH2 + O2 | Cavia porcellus | - |
17alpha-hydroxypregnenolone + A + H2O | - |
? | |
| 1.14.14.19 | pregnenolone + AH2 + O2 | Sus scrofa | - |
17alpha-hydroxypregnenolone + A + H2O | - |
? | |
| 1.14.14.19 | pregnenolone + AH2 + O2 | Bos taurus | - |
17alpha-hydroxypregnenolone + A + H2O | - |
? | |
| 1.14.14.19 | progesterone + AH2 + O2 | Cavia porcellus | - |
17alpha-hydroxyprogesterone + A + H2O | - |
? | |
| 1.14.14.19 | progesterone + AH2 + O2 | Sus scrofa | - |
17alpha-hydroxyprogesterone + A + H2O | - |
? | |
| 1.14.14.19 | progesterone + AH2 + O2 | Bos taurus | - |
17alpha-hydroxyprogesterone + A + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | Bos taurus | - |
- |
- |
| 1.14.14.19 | Cavia porcellus | - |
- |
- |
| 1.14.14.19 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.19 | recombinant fusion enzyme from Escherichia coli by 2',5'-ADP affinity chromatography | Cavia porcellus |
| 1.14.14.19 | recombinant fusion enzyme from Escherichia coli by 2',5'-ADP affinity chromatography | Sus scrofa |
| 1.14.14.19 | recombinant fusion enzyme from Escherichia coli by 2',5'-ADP affinity chromatography | Bos taurus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 0.058 | - |
purified recombinant enzyme, activity with progesterone | Cavia porcellus |
| 1.14.14.19 | 0.1 | - |
purified recombinant enzyme, activity with pregnenolone | Cavia porcellus |
| 1.14.14.19 | 0.1 | - |
purified recombinant enzyme, activity with pregnenolone | Sus scrofa |
| 1.14.14.19 | 0.125 | - |
purified recombinant enzyme, activity with progesterone | Sus scrofa |
| 1.14.14.19 | 0.28 | - |
purified recombinant enzyme, activity with pregnenolone | Bos taurus |
| 1.14.14.19 | 0.47 | - |
purified recombinant enzyme, activity with progesterone | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.19 | additional information | the enzyme catalyzes the key branch point in the metabolic conversion of cholesterol either to androgens and estrogens, or to glucocorticoids | Cavia porcellus | ? | - |
? | |
| 1.14.14.19 | additional information | the enzyme catalyzes the key branch point in the metabolic conversion of cholesterol either to androgens and estrogens, or to glucocorticoids | Sus scrofa | ? | - |
? | |
| 1.14.14.19 | additional information | the enzyme catalyzes the key branch point in the metabolic conversion of cholesterol either to androgens and estrogens, or to glucocorticoids | Bos taurus | ? | - |
? | |
| 1.14.14.19 | additional information | the bifunctional enzyme also exhibits 17,20-lyase activity converting 17alpha-hydroxypregnenolone to dehydroepiandrosterone-S, EC 4.1.2.30, overview | Cavia porcellus | ? | - |
? | |
| 1.14.14.19 | additional information | the bifunctional enzyme also exhibits 17,20-lyase activity converting 17alpha-hydroxypregnenolone to dehydroepiandrosterone-S, EC 4.1.2.30, overview | Sus scrofa | ? | - |
? | |
| 1.14.14.19 | additional information | the bifunctional enzyme also exhibits 17,20-lyase activity converting 17alpha-hydroxypregnenolone to dehydroepiandrosterone-S, EC 4.1.2.30, overview | Bos taurus | ? | - |
? | |
| 1.14.14.19 | pregnenolone + AH2 + O2 | - |
Cavia porcellus | 17alpha-hydroxypregnenolone + A + H2O | - |
? | |
| 1.14.14.19 | pregnenolone + AH2 + O2 | - |
Sus scrofa | 17alpha-hydroxypregnenolone + A + H2O | - |
? | |
| 1.14.14.19 | pregnenolone + AH2 + O2 | - |
Bos taurus | 17alpha-hydroxypregnenolone + A + H2O | - |
? | |
| 1.14.14.19 | progesterone + AH2 + O2 | - |
Cavia porcellus | 17alpha-hydroxyprogesterone + A + H2O | - |
? | |
| 1.14.14.19 | progesterone + AH2 + O2 | - |
Sus scrofa | 17alpha-hydroxyprogesterone + A + H2O | - |
? | |
| 1.14.14.19 | progesterone + AH2 + O2 | - |
Bos taurus | 17alpha-hydroxyprogesterone + A + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.19 | 17 alpha-hydroxylase/C17,20-lyase | - |
Cavia porcellus |
| 1.14.14.19 | 17 alpha-hydroxylase/C17,20-lyase | - |
Sus scrofa |
| 1.14.14.19 | 17 alpha-hydroxylase/C17,20-lyase | - |
Bos taurus |
| 1.14.14.19 | cytochrome P450c17 | - |
Cavia porcellus |
| 1.14.14.19 | cytochrome P450c17 | - |
Sus scrofa |
| 1.14.14.19 | cytochrome P450c17 | - |
Bos taurus |
| 1.14.14.19 | P450c17 | - |
Cavia porcellus |
| 1.14.14.19 | P450c17 | - |
Sus scrofa |
| 1.14.14.19 | P450c17 | - |
Bos taurus |
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