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Literature summary extracted from

  • Yamanaka, H.; Nakajima, M.; Fukami, T.; Sakai, H.; Nakamura, A.; Katoh, M.; Takamiya, M.; Aoki, Y.; Yokoi, T.
    CYP2A6 AND CYP2B6 are involved in nornicotine formation from nicotine in humans: interindividual differences in these contributions (2005), Drug Metab. Dispos., 33, 1811-1818.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.B11 expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.B11 anti-CYP2A6 antibody
-
 Homo sapiens
1.14.14.B11 coumarin
-
 Homo sapiens
1.14.14.B11 additional information not inhibitory: quinidine, anti-CYP2D6 serum, ketoconazole, anti-CYP3A4 serum Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.B11 0.049
-
 Nicotine isoform CYP2A6, pH 7.4, 37°C Homo sapiens
1.14.14.B11 0.55
-
 Nicotine isoform CYP2B6, pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.B11 microsome nicotine N-demethylase activity in microsomes from human livers at 0.02 mM nicotine is significantly correlated with the isoform CYP2A6 contents, while the nicotine N-demethylase activity at 0.1 mM nicotine is significantly correlated with the isoform CYP2B6 contents Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.B11 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.B11 liver nicotine N-demethylase activity in microsomes from human livers at 0.02 mM nicotine is significantly correlated with the isoform CYP2A6 contents, while the nicotine N-demethylase activity at 0.1 mM nicotine is significantly correlated with the isoform CYP2B6 contents Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.B11 additional information isoforms CYP2A6 and CYP2B6 significantly contribute to the nicotine N-demethylation at low and high substrate concentrations, respectively Homo sapiens ?
-
 ?
1.14.14.B11 nicotine + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.B11 cytochrome P450
-
 Homo sapiens