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Literature summary extracted from

  • Bae, I.K.; Lee, Y-N.; Jeong, S.H.; Hong, S.G.; Lee, J.H.; Lee, S.H.; Kim, H.J.; Youn, H.
    Genetic and biochemical characterization of GES-5, an extended-spectrum class A beta-lactamase from Klebsiella pneumoniae (2007), Diagn. Microbiol. Infect. Dis., 58, 465-468.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21 (DE3) cells Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0042
-
 Imipenem at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.341
-
 cefotaxime at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.37
-
 benzylpenicillin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.394
-
 ceftaxidime at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.454
-
 piperacillin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.506
-
 cephaloridine at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.577
-
 cephalothin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 0.65
-
 cefoxitin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Klebsiella pneumoniae Q09HD0
-
-
3.5.2.6 Klebsiella pneumoniae CHAK36 Q09HD0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 Ni-NTA column chromatography Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Klebsiella pneumoniae (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Klebsiella pneumoniae CHAK36 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Klebsiella pneumoniae (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Klebsiella pneumoniae CHAK36 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Klebsiella pneumoniae (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Klebsiella pneumoniae CHAK36 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftaxidime + H2O
-
 Klebsiella pneumoniae (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Klebsiella pneumoniae (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Klebsiella pneumoniae CHAK36 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Klebsiella pneumoniae (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Klebsiella pneumoniae CHAK36 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Klebsiella pneumoniae (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Klebsiella pneumoniae (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 GES-5 an extended-spectrum class A beta-lactamase Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.3
-
 ceftaxidime at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 1.2
-
 Imipenem at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 2.9
-
 cefotaxime at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 4
-
 piperacillin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 9.6
-
 cefoxitin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 49.7
-
 cephalothin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 190
-
 cephaloridine at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae
3.5.2.6 317
-
 benzylpenicillin at 30°C in 100 mM sodium phosphate buffer, pH 7.0 Klebsiella pneumoniae