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Literature summary extracted from

  • Hawkins, T.A.; Haramis, A.; Etard, C.; Prodromou, C.; Vaughan, C.K.; Ashworth, R.; Ray, S.; Behra, M.; Holder, N.; Talbot, W.S.; Pearl, L.H.; Strahle, U.; Wilson, S.W.
    The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis (2008), Development, 135, 1147-1156.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.7 full-length u45 mutant and wild-type hsp90a are amplified by RT-PCR, cloned into the the TOPO vector and subcloned by PCR into pCS2myc+ Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.10 additional information in slothu45 mutant, the initial steps in sarcomere assembly take place, but thick filaments are absent and filamentous I-Z-I brushes fail to align or adopt correct spacing. The mutation only affects skeletal muscle and mutant embryos show no other obvious phenotypes. Phenotype is due to mutation in one copy of a tandemly duplicated hsp90a gene disrupting the chaperoning function through interference with aTPase activity. Loss of Hsp90a function leads to the downregulation of genes encoding sarcomeric proteins and upregulation of hsp90a and several other genes encoding proteins that may act with Hsp90a during sarcomere assembly Danio rerio
5.6.1.7 slou45 the ATPase activity of Hsp90 is abrogated by the slou45 mutation Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide Danio rerio
-
 ADP + phosphate + an unfolded polypeptide
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.10 Danio rerio
-
 isoform Hsp90a
-
5.6.1.7 Danio rerio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.7 protein is extracted by homogenisation of deyolked embryos Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.7 embryo
-
 Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide
-
 Danio rerio ADP + phosphate + an unfolded polypeptide
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.6.4.10 Hsp90a
-
 Danio rerio
5.6.1.7 Hsp90a
-
 Danio rerio