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Literature summary extracted from

  • Fields, P.A.; Strothers, C.M.; Mitchell, M.A.
    Function of muscle-type lactate dehydrogenase and citrate synthase of the Galapagos marine iguana, Amblyrhynchus cristatus, in relation to temperature (2008), Comp. Biochem. Physiol. B, 150, 62-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 gene ldh-a, DNA and amino acid sequence determination and analysis Amblyrhynchus cristatus
1.1.1.27 gene ldh-a, DNA and amino acid sequence determination and analysis Iguana iguana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.27 I283V | site-directed mutagenesis, the mutation switches the nature of the residue from Amblyrhynchus cristatus to that of Iguana iguana, another Galapagos marine iguana, the mutation does not affect enzyme kinetics, overview Amblyrhynchus cristatus
1.1.1.27 T9A site-directed mutagenesis, the mutation switches the nature of the residue from Amblyrhynchus cristatus to that of Iguana iguana, another Galapagos marine iguana, the mutation does not affect enzyme kinetics, overview Amblyrhynchus cristatus
1.1.1.27 T9A/I283V site-directed mutagenesis, the mutations switch the nature of the residues from Amblyrhynchus cristatus to those of Iguana iguana, another Galapagos marine iguana, the mutation affects enzyme kinetics decreasing Km for pyruvate and kcat, overview Amblyrhynchus cristatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ Amblyrhynchus cristatus
-
 pyruvate + NADH + H+
-
 r
1.1.1.27 (S)-lactate + NAD+ Iguana iguana
-
 pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Amblyrhynchus cristatus Q0GND9 Galapagos marine iguana, gene ldh-a
-
1.1.1.27 Iguana iguana Q0GND8 common iguana
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.27 skeletal muscle muscle isozyme Amblyrhynchus cristatus
-
1.1.1.27 skeletal muscle muscle isozyme Iguana iguana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
 Amblyrhynchus cristatus pyruvate + NADH + H+
-
 r
1.1.1.27 (S)-lactate + NAD+
-
 Iguana iguana pyruvate + NADH + H+
-
 r
1.1.1.27 additional information amino acid sequence, structure and kinetic comparison of L-LDH with citrate synthase, EC 2.3.3.1, overview Amblyrhynchus cristatus ?
-
 ?
1.1.1.27 additional information amino acid sequence, structure and kinetic comparison of L-LDH with citrate synthase, EC 2.3.3.1, overview Iguana iguana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 More molecular modeling of the three-dimensional enzyme structure in comparison to the enzyme structure from Amblyrhynchus cristatus, overview Iguana iguana
1.1.1.27 More molecular modeling of the three-dimensional enzyme structure in comparison to the enzyme structure from Iguana iguana, overview Amblyrhynchus cristatus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 A4-LDH
-
 Amblyrhynchus cristatus
1.1.1.27 A4-LDH
-
 Iguana iguana
1.1.1.27 anaerobic lactate dehydrogenase
-
 Amblyrhynchus cristatus
1.1.1.27 anaerobic lactate dehydrogenase
-
 Iguana iguana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 30
-
 assay at Amblyrhynchus cristatus
1.1.1.27 30
-
 assay at Iguana iguana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.27 14 46 in vivo temperature Amblyrhynchus cristatus
1.1.1.27 14 46 in vivo temperature Iguana iguana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 7
-
 assay at Amblyrhynchus cristatus
1.1.1.27 7
-
 assay at Iguana iguana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Amblyrhynchus cristatus
1.1.1.27 NAD+
-
 Iguana iguana
1.1.1.27 NADH
-
 Amblyrhynchus cristatus
1.1.1.27 NADH
-
 Iguana iguana