BRENDA - Enzyme Database

Purification, characterization, and cDNA cloning of opine dehydrogenases from the polychaete rockworm Marphysa sanguinea

Endo, N.; Kan-No, N.; Nagahisa, E.; Comp. Biochem. Physiol. B 147B, 293-307 (2007)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.5.1.22
expressed in Escherichia coli
Marphysa sanguinea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.22
0.07
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.85
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.87
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.96
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.97
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
9.07
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
9.17
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
10.2
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
11.71
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
12.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
39.8
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
40.1
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
382.1
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.22
40600
-
reduced and denatured enzyme, estimated by SDS-PAGE
Marphysa sanguinea
1.5.1.22
42000
-
native enzyme, estimated by SDS-PAGE
Marphysa sanguinea
1.5.1.22
43350
-
deduced from amino acid sequence from strombine/alanopine dehydrogenase
Marphysa sanguinea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.22
Marphysa sanguinea
A0A9I9
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.5.1.22
ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography
Marphysa sanguinea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.22
muscle
-
Marphysa sanguinea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5.1.22
2.6
-
crude extract, at 30°C
Marphysa sanguinea
1.5.1.22
87
-
after 29fold purification, at 30°C
Marphysa sanguinea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.22
Gly + 2-ketobutyrate + NADH + H+
8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + glyoxylate + NADH + H+
3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + oxaloacetate + NADH + H+
39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-carboxymethylglycine + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + pyruvate + NADH + H+
94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + pyruvate + NADPH
less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NADP+ + H2O
-
-
-
?
1.5.1.22
L-Ala + 2-ketobutyrate + NADH + H+
8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + glyoxylate + NADH + H+
4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(carboxymethyl)-L-Ala + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + oxaloacetate + NADH + H+
40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + pyruvate + NADH + H+
100% activity
686211
Marphysa sanguinea
meso-alanopine + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + pyruvate + NADPH
less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
meso-alanopine + NADP+ + H2O
-
-
-
?
1.5.1.22
L-Cys + pyruvate + NADH + H+
1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ser + pyruvate + NADH + H+
14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Val + pyruvate + NADH + H+
0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Val + NAD+ + H2O
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5.1.22
3.86
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
3.96
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
4.99
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
5.12
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
21.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
23.2
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
23.64
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
42.66
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
43.58
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
44.25
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
44.7
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
47.33
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
47.78
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
50.08
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
51.7
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.22
NAD+
-
Marphysa sanguinea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.22
expressed in Escherichia coli
Marphysa sanguinea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.22
NAD+
-
Marphysa sanguinea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.22
0.07
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.85
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.87
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.96
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
0.97
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
9.07
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
9.17
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
10.2
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
11.71
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
12.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
39.8
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
40.1
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
382.1
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.22
40600
-
reduced and denatured enzyme, estimated by SDS-PAGE
Marphysa sanguinea
1.5.1.22
42000
-
native enzyme, estimated by SDS-PAGE
Marphysa sanguinea
1.5.1.22
43350
-
deduced from amino acid sequence from strombine/alanopine dehydrogenase
Marphysa sanguinea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.22
ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography
Marphysa sanguinea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.22
muscle
-
Marphysa sanguinea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5.1.22
2.6
-
crude extract, at 30°C
Marphysa sanguinea
1.5.1.22
87
-
after 29fold purification, at 30°C
Marphysa sanguinea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.22
Gly + 2-ketobutyrate + NADH + H+
8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + glyoxylate + NADH + H+
3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + oxaloacetate + NADH + H+
39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-carboxymethylglycine + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + pyruvate + NADH + H+
94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NAD+ + H2O
-
-
-
?
1.5.1.22
Gly + pyruvate + NADPH
less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NADP+ + H2O
-
-
-
?
1.5.1.22
L-Ala + 2-ketobutyrate + NADH + H+
8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + glyoxylate + NADH + H+
4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(carboxymethyl)-L-Ala + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + oxaloacetate + NADH + H+
40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + pyruvate + NADH + H+
100% activity
686211
Marphysa sanguinea
meso-alanopine + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ala + pyruvate + NADPH
less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
meso-alanopine + NADP+ + H2O
-
-
-
?
1.5.1.22
L-Cys + pyruvate + NADH + H+
1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Ser + pyruvate + NADH + H+
14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
-
-
-
?
1.5.1.22
L-Val + pyruvate + NADH + H+
0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Val + NAD+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5.1.22
3.86
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
3.96
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
4.99
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
5.12
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
1.5.1.22
21.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
23.2
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
23.64
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
42.66
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
43.58
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
44.25
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
44.7
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
47.33
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
47.78
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
50.08
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
1.5.1.22
51.7
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea