EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.22 | expressed in Escherichia coli | Marphysa sanguinea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.22 | 0.07 | - |
NADH | apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 0.07 | - |
NADH | apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 0.85 | - |
NAD+ | apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 0.87 | - |
NAD+ | apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 0.96 | - |
pyruvate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 0.97 | - |
pyruvate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 9.07 | - |
meso-Alanopine | apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 9.17 | - |
D-strombine | apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 10.2 | - |
oxaloacetate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 10.2 | - |
oxaloacetate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 11.71 | - |
2-Ketobutyrate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 12.77 | - |
2-Ketobutyrate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 39.8 | - |
L-Ala | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 40.1 | - |
Gly | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 382.1 | - |
L-Ser | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 40600 | - |
reduced and denatured enzyme, estimated by SDS-PAGE | Marphysa sanguinea |
1.5.1.22 | 42000 | - |
native enzyme, estimated by SDS-PAGE | Marphysa sanguinea |
1.5.1.22 | 43350 | - |
deduced from amino acid sequence from strombine/alanopine dehydrogenase | Marphysa sanguinea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.22 | Marphysa sanguinea | A0A9I9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.22 | ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography | Marphysa sanguinea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.22 | muscle | - |
Marphysa sanguinea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 2.6 | - |
crude extract, at 30°C | Marphysa sanguinea |
1.5.1.22 | 87 | - |
after 29fold purification, at 30°C | Marphysa sanguinea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.22 | Gly + 2-ketobutyrate + NADH + H+ | 8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | 2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O | - |
? | |
1.5.1.22 | Gly + glyoxylate + NADH + H+ | 3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | [(carboxymethyl)amino]propanedioic acid + NAD+ + H2O | - |
? | |
1.5.1.22 | Gly + oxaloacetate + NADH + H+ | 39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | N-carboxymethylglycine + NAD+ + H2O | - |
? | |
1.5.1.22 | Gly + pyruvate + NADH + H+ | 94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | D-strombine + NAD+ + H2O | - |
? | |
1.5.1.22 | Gly + pyruvate + NADPH | less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | D-strombine + NADP+ + H2O | - |
? | |
1.5.1.22 | L-Ala + 2-ketobutyrate + NADH + H+ | 8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | 2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O | - |
? | |
1.5.1.22 | L-Ala + glyoxylate + NADH + H+ | 4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | N-(carboxymethyl)-L-Ala + NAD+ + H2O | - |
? | |
1.5.1.22 | L-Ala + oxaloacetate + NADH + H+ | 40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | [[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O | - |
? | |
1.5.1.22 | L-Ala + pyruvate + NADH + H+ | 100% activity | Marphysa sanguinea | meso-alanopine + NAD+ + H2O | - |
? | |
1.5.1.22 | L-Ala + pyruvate + NADPH | less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | meso-alanopine + NADP+ + H2O | - |
? | |
1.5.1.22 | L-Cys + pyruvate + NADH + H+ | 1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | N-(1-carboxyethyl)-L-Cys + NAD+ + H2O | - |
? | |
1.5.1.22 | L-Ser + pyruvate + NADH + H+ | 14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | N-(1-carboxyethyl)-L-Ser + NAD+ + H2O | - |
? | |
1.5.1.22 | L-Val + pyruvate + NADH + H+ | 0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 | Marphysa sanguinea | N-(1-carboxyethyl)-L-Val + NAD+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.22 | St/AlDH1 | isozyme | Marphysa sanguinea |
1.5.1.22 | St/AlDH2 | isozyme | Marphysa sanguinea |
1.5.1.22 | St/AlDH3 | isozyme | Marphysa sanguinea |
1.5.1.22 | strombine/alanopine dehydrogenase | St/AlDH functions physiologically as strombine dehydrogenase | Marphysa sanguinea |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.22 | 3.86 | - |
NAD+ | apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 3.96 | - |
NAD+ | apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 4.99 | - |
D-strombine | apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 5.12 | - |
meso-Alanopine | apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 21.77 | - |
2-Ketobutyrate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 23.2 | - |
2-Ketobutyrate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 23.64 | - |
L-Ser | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 42.66 | - |
oxaloacetate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 43.58 | - |
NADH | apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 44.25 | - |
oxaloacetate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 44.7 | - |
NADH | apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 47.33 | - |
pyruvate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 47.78 | - |
pyruvate | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 50.08 | - |
Gly | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea | |
1.5.1.22 | 51.7 | - |
L-Ala | apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C | Marphysa sanguinea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.22 | NAD+ | - |
Marphysa sanguinea |