Literature summary extracted from

Sun, S.; Gui, Y.; Wang, Y.; Qian, L.; Jiang, Q.; Liu, D.; Song, H.
Effect of dihydrofolate reductase gene knock-down on the expression of heart and neural crest derivatives expressed transcript 2 in zebrafish cardiac development (2007), Chin. Med. J., 120, 1166-1171.

Application

EC Number	Application	Comment	Organism
1.5.1.3	medicine	knock-down of dihydrofolate reductase or heart and neural crest derivatives expressed transcript HAND2 in fertilized eggs causes cardiac malformation. Expression of HAND2 is reduced in dihydrofolate reductase knock-down embryos. Microinjection of HAND2 mRNA into fertilized eggs can induce HAND2 overexpression which rescues the cardiac malformation phenotypes of dihydrofolate knock-down embryos	Danio rerio

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.5.1.3	additional information	knock-down of dihydrofolate reductase or heart and neural crest derivatives expressed transcript HAND2 in fertilized eggs causes cardiac malformation. Expression of HAND2 is reduced in dihydrofolate reductase knock-down embryos. Microinjection of HAND2 mRNA into fertilized eggs can induce HAND2 overexpression which rescues the cardiac malformation phenotypes of dihydrofolate knock-down embryos	Danio rerio

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.3	Danio rerio	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.5.1.3	embryo	knock-down of dihydrofolate reductase or heart and neural crest derivatives expressed transcript HAND2 in fertilized eggs causes cardiac malformation. Expression of HAND2 is reduced in dihydrofolate reductase knock-down embryos. Microinjection of HAND2 mRNA into fertilized eggs can induce HAND2 overexpression which rescues the cardiac malformation phenotypes of dihydrofolate knock-down embryos	Danio rerio	-
1.5.1.3	heart	knock-down of dihydrofolate reductase or heart and neural crest derivatives expressed transcript HAND2 in fertilized eggs causes cardiac malformation	Danio rerio	-

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Release 2023.1 (January 2023)