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Literature summary extracted from

  • Rabe, K.S.; Kiko, K.; Niemeyer, C.M.
    Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius (2008), Chembiochem, 9, 420-425.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.7 expressed in Escherichia coli BL21 (DE3) cells Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Sulfolobus acidocaldarius Q55080 DSM 639
-
1.11.1.7 Sulfolobus acidocaldarius DSM 639 Q55080 DSM 639
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 Ni-NTA column chromatography and MonoQ column chromatography Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 AmplexRed + cumene hydroperoxide the initial activity of CYP119 in the presence of cumene hydroperoxide is 5fold higher than that observed with H2O2 Sulfolobus acidocaldarius ?
-
 ?
1.11.1.7 AmplexRed + cumene hydroperoxide the initial activity of CYP119 in the presence of cumene hydroperoxide is 5fold higher than that observed with H2O2 Sulfolobus acidocaldarius DSM 639 ?
-
 ?
1.11.1.7 AmplexRed + H2O2
-
 Sulfolobus acidocaldarius ? + H2O
-
 ?
1.11.1.7 AmplexRed + H2O2
-
 Sulfolobus acidocaldarius DSM 639 ? + H2O
-
 ?
1.11.1.7 AmplexRed + tert-butyl hydroperoxide the initial activity of CYP119 in the presence of tert-butyl hydroperoxide is 2fold higher than that observed with H2O2 Sulfolobus acidocaldarius ?
-
 ?
1.11.1.7 AmplexRed + tert-butyl hydroperoxide the initial activity of CYP119 in the presence of tert-butyl hydroperoxide is 2fold higher than that observed with H2O2 Sulfolobus acidocaldarius DSM 639 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 CYP119
-
 Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 75
-
 high reactivity at 75°C Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 8.5
-
-
 Sulfolobus acidocaldarius