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Literature summary extracted from

  • Kudryashova, E.V.; Visser, A.J.; van Berkel, W.J.
    Monomer formation and function of p-hydroxybenzoate hydroxylase in reverse micelles and in dimethylsulfoxide/water mixtures (2008), Chembiochem, 9, 413-419.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.2 0.0006
-
 4-hydroxybenzoate pH 8, monomeric form Pseudomonas fluorescens
1.14.13.2 0.025
-
 NADPH pH 8, dimeric form Pseudomonas fluorescens
1.14.13.2 0.025
-
 4-hydroxybenzoate pH 8, dimeric form Pseudomonas fluorescens
1.14.13.2 0.052
-
 NADPH pH 8, monomeric form Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.2 dimer the monomeric form of PHBH, which is not achieved under conventional conditions, is isolated by entrapment in reverse micelles and by addition of DMSO. The PHBH monomer is catalytically more efficient than the PHBH dimer Pseudomonas fluorescens
1.14.13.2 monomer the monomeric form of PHBH, which is not achieved under conventional conditions, is isolated by entrapment in reverse micelles and by addition of DMSO. The PHBH monomer is catalytically more efficient than the PHBH dimer Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.14.13.2 p-hydroxybenzoate hydroxylase
-
 Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.2 45
-
 NADPH pH 8, monomeric form Pseudomonas fluorescens
1.14.13.2 45
-
 4-hydroxybenzoate pH 8, monomeric form Pseudomonas fluorescens
1.14.13.2 63
-
 NADPH pH 8, dimeric form Pseudomonas fluorescens
1.14.13.2 63
-
 4-hydroxybenzoate pH 8, dimeric form Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 NADPH
-
 Pseudomonas fluorescens