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Literature summary extracted from

  • Namdjou, D.J.; Chen, H.M.; Vinogradov, E.; Brochu, D.; Withers, S.G.; Wakarchuk, W.W.
    A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis (2008), Chembiochem, 9, 1632-1640.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.38 synthesis the enzyme is useful as catalyst for quantitative synthesis of Gal-beta-1,4-Man-pNP, overview Neisseria meningitidis
2.4.1.38 synthesis the enzyme is useful as catalyst for quantitative synthesis of the thiodisaccharide Gal-betaS-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP, overview Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 expression of full-length HP0826 in Escherichia coli Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 additional information
-
 additional information kinetics Neisseria meningitidis
2.4.1.38 additional information
-
 additional information kinetics Helicobacter pylori
2.4.1.38 0.27
-
 UDP-Gal pH 7.2 Helicobacter pylori
2.4.1.38 0.79
-
 pNP-beta-GlcNAc pH 7.2 Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Mn2+ activates Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.38 additional information Helicobacter pylori HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori ?
-
 ?
2.4.1.38 additional information Neisseria meningitidis LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Helicobacter pylori
-
-
-
2.4.1.38 Neisseria meningitidis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.38 160
-
 purified recombinant enzyme Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 additional information HP0826 is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori Helicobacter pylori ?
-
 ?
2.4.1.38 additional information LgtB is the beta-1,4-galactosyltransferase involved in the biosynthesis of the lipopolysaccharide O-chain backbone of Helicobacter pylori Neisseria meningitidis ?
-
 ?
2.4.1.38 additional information HP0826 exhibits a pronounced acceptor promiscuity with the most striking result being the successful transfer of Gal to the 4-SH of pNP-4-S-beta-GlcNAc Helicobacter pylori ?
-
 ?
2.4.1.38 UDP-Gal + 4-nitrophenyl 2-acetamido-2-deoxy-4-thio-beta-D-glucopyranoside HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview Helicobacter pylori UDP + Gal-beta-S-1,4-GlcNAcpNP
-
 ?
2.4.1.38 UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside HP0826 catalyzes the synthesis of the thiodisaccharide Gal-beta-S-1,4-GlcNAcpNP as well as Gal-beta-1,4-Man-pNP with total regio- and stereoselectivity, overview Helicobacter pylori UDP + Gal-beta-1,4-Man-pNP
-
 ?
2.4.1.38 UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside LgtB catalyzes the synthesis of Gal-beta-1,4-Man-pNP, overview Neisseria meningitidis UDP + Gal-beta-1,4-Man-pNP
-
 ?
2.4.1.38 UDP-Gal + 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Helicobacter pylori UDP + ?
-
 ?
2.4.1.38 UDP-galactose + p-nitrophenyl-beta-D-N-acetylglucosaminide
-
 Helicobacter pylori ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.38 beta-1,4-galactosyltransferase
-
 Neisseria meningitidis
2.4.1.38 beta-1,4-galactosyltransferase
-
 Helicobacter pylori
2.4.1.38 HP0826
-
 Helicobacter pylori
2.4.1.38 LgtB
-
 Neisseria meningitidis
2.4.1.38 More HP0826 belongs to the carbohydrate active enzyme family GT25 of inverting glycosyltransferases Helicobacter pylori
2.4.1.38 More LgtB belongs to the carbohydrate active enzyme family GT25 of inverting glycosyltransferases Neisseria meningitidis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 7.2
-
-
 Helicobacter pylori