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Literature summary extracted from

  • Cui, X.; Wang, Y.; Guo, J.
    Osmotic regulation of betaine content in Leymus chinensis under saline-alkali stress and cloning and expression of betaine aldehyde dehydrogenase (BADH) gene (2008), Chem. Res. Chin. Univ., 24, 204-209.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.8 propan-2-yl 1-thio-beta-D-galactopyranoside for the expressed protein, final concentration of 1 mmol in culture solution Leymus chinensis

Application

EC Number Application Comment Organism
1.2.1.8 agriculture potential: the BADH gene can be cloned from Leymus chinensis under stress treatment, which indicates that its expression and regulation may play an important role in stress tolerance. Therefore, it can be transformed into other plants to obtain transgenic species with a high saline-alkali tolerance by biotechnology; as a result, it can speed up the recovering and rebuilding of saline-alkaline grassland Leymus chinensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 the BADH gene of Leymus chinensis is cloned by RT-PCR and RACE technology and is designated as LcBADH. The cDNA sequence of LcBADH is 1774 bp including the open reading frame of 1521 bp (coding 506 amino acids). The vector of prokaryotic expression is constructed by inserting the LcBADH gene fragment into pET30a(+) and transformed into Escherichia coli BL21(DE3) Leymus chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.8 peroxisome putative Leymus chinensis 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.8 Cl- treatment of seedlings after germinating with 6.25-200 mmol/l Leymus chinensis
1.2.1.8 Na+ treatment of seedlings after germinating with 18.75-400 mmol/l Leymus chinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 56780
-
mass of expressed protein (LcBADH) determinating by SDS-PAGE Leymus chinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Leymus chinensis
-
betaine + NADH + 2 H+ accumulation of betaine is a strategy of plants to survive drought, salinity, and extreme temperatures ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Leymus chinensis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.8 betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ catalyzes the last step of the two-step oxidation Leymus chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.8 seedling
-
Leymus chinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 0.00826
-
specific activity of expressed protein after 3 h IPTG induction. After disruption of the cells by ultrasonication, supernatant is used to assay the enzyme activity. Reaction is started by adding 0.05 ml of 10 mmol/l betaine aldehyde chloride at 30°C and lasted for 30 min. Specific activity is detected in crude enzyme extracts. BADH activity from induced bacteria is 75.3% higher than in the control. Leymus chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
Leymus chinensis betaine + NADH + 2 H+ accumulation of betaine is a strategy of plants to survive drought, salinity, and extreme temperatures ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
Leymus chinensis
1.2.1.8 betaine aldehyde dehydrogenase
-
Leymus chinensis
1.2.1.8 LcBADH
-
Leymus chinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.8 30
-
assay at Leymus chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 9.6
-
assay at Leymus chinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.8 7.8 10.1 assay at Leymus chinensis