Literature summary extracted from
Cui, X.; Wang, Y.; Guo, J.
Osmotic regulation of betaine content in Leymus chinensis under saline-alkali stress and cloning and expression of betaine aldehyde dehydrogenase (BADH) gene (2008), Chem. Res. Chin. Univ., 24, 204-209.
No PubMed abstract available
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.2.1.8 |
propan-2-yl 1-thio-beta-D-galactopyranoside |
for the expressed protein, final concentration of 1 mmol in culture solution |
Leymus chinensis |
|
Application
EC Number |
Application |
Comment |
Organism |
---|
1.2.1.8 |
agriculture |
potential: the BADH gene can be cloned from Leymus chinensis under stress treatment, which indicates that its expression and regulation may play an important role in stress tolerance. Therefore, it can be transformed into other plants to obtain transgenic species with a high saline-alkali tolerance by biotechnology; as a result, it can speed up the recovering and rebuilding of saline-alkaline grassland |
Leymus chinensis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.2.1.8 |
the BADH gene of Leymus chinensis is cloned by RT-PCR and RACE technology and is designated as LcBADH. The cDNA sequence of LcBADH is 1774 bp including the open reading frame of 1521 bp (coding 506 amino acids). The vector of prokaryotic expression is constructed by inserting the LcBADH gene fragment into pET30a(+) and transformed into Escherichia coli BL21(DE3) |
Leymus chinensis |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.2.1.8 |
peroxisome |
putative |
Leymus chinensis |
5777 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.2.1.8 |
Cl- |
treatment of seedlings after germinating with 6.25-200 mmol/l |
Leymus chinensis |
|
1.2.1.8 |
Na+ |
treatment of seedlings after germinating with 18.75-400 mmol/l |
Leymus chinensis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.2.1.8 |
56780 |
- |
mass of expressed protein (LcBADH) determinating by SDS-PAGE |
Leymus chinensis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.2.1.8 |
betaine aldehyde + NAD+ + H2O |
Leymus chinensis |
- |
betaine + NADH + 2 H+ |
accumulation of betaine is a strategy of plants to survive drought, salinity, and extreme temperatures |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.2.1.8 |
Leymus chinensis |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.2.1.8 |
betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ |
catalyzes the last step of the two-step oxidation |
Leymus chinensis |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.2.1.8 |
seedling |
- |
Leymus chinensis |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.2.1.8 |
0.00826 |
- |
specific activity of expressed protein after 3 h IPTG induction. After disruption of the cells by ultrasonication, supernatant is used to assay the enzyme activity. Reaction is started by adding 0.05 ml of 10 mmol/l betaine aldehyde chloride at 30°C and lasted for 30 min. Specific activity is detected in crude enzyme extracts. BADH activity from induced bacteria is 75.3% higher than in the control. |
Leymus chinensis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.2.1.8 |
betaine aldehyde + NAD+ + H2O |
- |
Leymus chinensis |
betaine + NADH + 2 H+ |
accumulation of betaine is a strategy of plants to survive drought, salinity, and extreme temperatures |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.2.1.8 |
BADH |
- |
Leymus chinensis |
1.2.1.8 |
betaine aldehyde dehydrogenase |
- |
Leymus chinensis |
1.2.1.8 |
LcBADH |
- |
Leymus chinensis |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
1.2.1.8 |
30 |
- |
assay at |
Leymus chinensis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.2.1.8 |
9.6 |
- |
assay at |
Leymus chinensis |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
1.2.1.8 |
7.8 |
10.1 |
assay at |
Leymus chinensis |