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Literature summary extracted from

  • Krahulec, S.; Armao, G.C.; Weber, H.; Klimacek, M.; Nidetzky, B.
    Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate (2008), Carbohydr. Res., 343, 1414-1423.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.17 synthesis hydrogen transfer from formate to D-fructose 6-phosphate, mediated by NAD(H) and catalyzed by a coupled enzyme system of purified Candida boidinii formate dehydrogenase and AfM1PDH, is used for the preparative synthesis of D-mannitol 1-phosphate or, by applying an analogous procedure using deuterio formate, the 5-[2H] derivative thereof, overview Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 high level overexpression in Escherichia coli strain JM109 Aspergillus fumigatus
1.17.1.9 expressed in Escherichia coli JM109 cells [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 additional information
-
 additional information kinetic isotope effects, overview Aspergillus fumigatus
1.1.1.17 0.016
-
 NADH pH 7.1, 25°C, recombinant enzyme Aspergillus fumigatus
1.1.1.17 0.23
-
 D-mannitol 1-phosphate pH 7.1, 25°C, recombinant enzyme Aspergillus fumigatus
1.1.1.17 0.75
-
 NAD+ pH 7.1, 25°C, recombinant enzyme Aspergillus fumigatus
1.1.1.17 2.1
-
 D-fructose 6-phosphate pH 7.1, 25°C, recombinant enzyme Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 40000
-
 x * 40000, recombinant enzyme, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Aspergillus fumigatus
-
 D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Aspergillus fumigatus Q4X1A4
-
-
1.17.1.9 [Candida] boidinii O13437 strain ATCC 18810
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 recombinant enzyme 2.1fold from Escherichia coli by affinity chromatography Aspergillus fumigatus
1.17.1.9 partial purification, Q-Sepharose column chromatography [Candida] boidinii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.17 D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+ the hydride transfer is strongly rate-determining for the overall enzymatic reaction Aspergillus fumigatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 17.6
-
 oxidation reaction, purified recombinant enzyme Aspergillus fumigatus
1.1.1.17 169
-
 reduction reaction, purified recombinant enzyme Aspergillus fumigatus
1.17.1.9 1
-
 crude extract, in 30 mM Tris-HCl, pH 7.5. at 25°C [Candida] boidinii
1.17.1.9 4.4
-
 recombinant enzyme, after 4.5fold purification, in 30 mM Tris-HCl, pH 7.5. at 25°C [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Aspergillus fumigatus D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ M1PDH is stereospecific for transferring the hydrogen to NAD+, in situ proton NMR studies of enzymatic oxidation of D-5-[2H]-mannitol 1-phosphate, overview Aspergillus fumigatus D-fructose 6-phosphate + NADH + H+
-
 r
1.17.1.9 formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.17 ? x * 40000, recombinant enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 long-chain mannitol-1-phosphate 5-dehydrogenase
-
 Aspergillus fumigatus
1.1.1.17 M1PDH
-
 Aspergillus fumigatus
1.17.1.9 FDH
-
 [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 25
-
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.17 8.5
-
 D-mannitol 1-phosphate pH 7.1, 25°C, recombinant enzyme Aspergillus fumigatus
1.1.1.17 125
-
 D-fructose 6-phosphate pH 7.1, 25°C, recombinant enzyme Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 7.1
-
-
 Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 additional information poor activity with NADP(H) Aspergillus fumigatus
1.1.1.17 NAD+ dependent on Aspergillus fumigatus
1.1.1.17 NADH dependent on Aspergillus fumigatus
1.17.1.9 NAD+
-
 [Candida] boidinii