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Literature summary extracted from

  • Mori, N.; Glunde, K.; Takagi, T.; Raman, V.; Bhujwalla, Z.M.
    Choline kinase down-regulation increases the effect of 5-fluorouracil in breast cancer cells (2007), Cancer Res., 67, 11284-11290.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.32 drug development choline kinase is elevated in cancer cells and presents a novel target for increasing cell kill. Data support exploring transient Chk silencing as a broad-spectrum chemotherapeutic agent for cancer cells. Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 Small interfering RNA-chk (siRNA) is designed and transfected for 48 h using oligofectamine and Opti-MEM based on the observed decrease of Chk message using reverse transcription-PCR analysis within this time. Immunoblot assay results with Chk antibody shows significant reduction of Chk protein expression levels in MCF-12A, MCF-7, and MDA-MB-231 cells after transient siRNA-chk transfection. Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 5-fluorouracil Investigation of the effect of combining transient siRNA-chk transfection with the anticancer drug 5-fluorouracil on cell viability and proliferation. Choline kinase down-regulation with 5-fluorouracil treatment increased cell kill in cancer cells. Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.32 additional information Homo sapiens microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 MCF-12A cell HMEC cell line, a spontaneously immortalized nonmalignant mammary epithelial cell line Homo sapiens
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2.7.1.32 MCF-7 cell estrogen receptor-positive poorly metastatic breast cancer cell line Homo sapiens
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2.7.1.32 MDA-MB-231 cell invasive estrogen receptor-negative cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 additional information microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 CHK
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 Homo sapiens
2.7.1.32 choline kinase
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 Homo sapiens