Any feedback?
Literature summary extracted from
- Engineered herpes simplex virus expressing bacterial cytosine deaminase for experimental therapy of brain tumors (2007), Cancer Gene Ther., 14, 45-56.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | expression of the enzyme in human and murine cancer cell lines and tumors using a herpes simplex type 1 virus, HSV-1, by exchanging the viral gamma1_34.5 gene for the cytosine deaminase CD gene, method development for the oncolytic HSV vector in gene therapy, overview | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | 5-fluorocytosine + H2O | direct inoculation of murine and human brain tumor cell lines, murine neuroblastoma and human glioma cells, with the enzyme transmitted in a recombinant HSV-1 viral oncolytic vector results in cytotoxicity and destruction of cancer cells wth increased cytotoxicity for neighbouring cells, overview | Escherichia coli | 5-fluorouracil + NH3 | - |
? |  |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 7.8 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
