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Literature summary extracted from

  • Liu, J.; Harada, H.; Ogura, M.; Shibata, T.; Hiraoka, M.
    Adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts (2007), Br. J. Cancer, 96, 1871-1878.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.4.1 medicine establishment of a hypoxia-dependent prodrug-activating system using the enzyme cloned into a hypoxia-targeted adenoviral vector, overview Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.1 expression in human cervical epithelial adenocarcinoma cell line HeLa, human pancreatic carcinoma cell line MIA PaCa-2, and human colon carcinoma cell lines WiDr and HT29 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.1 additional information adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O Escherichia coli recombinant enzyme encoded in an adenoviral vector expressed in murine cancers or human cancer cell lines for prodrug cancer gene therapy, Ad/5HREp-BCD-mediated cytotoxicity under hypoxic conditions, overview 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?
3.5.4.1 additional information Escherichia coli adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts of murine tumors, e.g. in human cervical epithelial adenocarcinoma cell line HeLa and human pancreatic carcinoma cell line MIA PaCa-2, as well as in human colon carcinoma cell lines WiDr and HT29, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli
-
 gene codA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O
-
 Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 5-fluorocytosine + H2O recombinant enzyme encoded in an adenoviral vector expressed in murine cancers or human cancer cell lines for prodrug cancer gene therapy, Ad/5HREp-BCD-mediated cytotoxicity under hypoxic conditions, overview Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
3.5.4.1 additional information adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts of murine tumors, e.g. in human cervical epithelial adenocarcinoma cell line HeLa and human pancreatic carcinoma cell line MIA PaCa-2, as well as in human colon carcinoma cell lines WiDr and HT29, overview Escherichia coli ?
-
 ?