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Literature summary extracted from

  • Nascimento, A.S.; Catalano-Dupuy, D.L.; Bernardes, A.; Neto, M.d.e..O.; Santos, M.A.; Ceccarelli, E.A.; Polikarpov, I.
    Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+ (2007), BMC Struct. Biol., 7, 69.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli Leptospira interrogans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.2 using 27% PEG 3350, 50 mM Tris-HCl buffer pH 7.0, at 18°C Leptospira interrogans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0195
-
 NADPH in 50 mM Tris-HCl, pH 8, at 30°C Leptospira interrogans

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Leptospira interrogans Q8EY89
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 Ni-NTA affinity chromatography Leptospira interrogans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 reduced ferredoxin + NADP+
-
 Leptospira interrogans oxidized ferredoxin + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer X-ray scattering Leptospira interrogans

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP(H) reductase
-
 Leptospira interrogans
1.18.1.2 ferredoxin-NADP+ reductase
-
 Leptospira interrogans
1.18.1.2 FNR
-
 Leptospira interrogans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 258
-
 NADPH in 50 mM Tris-HCl, pH 8, at 30°C Leptospira interrogans

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD
-
 Leptospira interrogans
1.18.1.2 NADP+
-
 Leptospira interrogans