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Literature summary extracted from

  • Castillo, B.; Bansal, V.; Ganesan, A.; Halling, P.; Secundo, F.; Ferrer, A.; Griebenow, K.; Barletta, G.
    On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg (2006), BMC Biotechnol., 6, 51.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 1,4-dioxane reduces the enzyme activity. Structural changes, flexibility, hydration, and changes of the enzyme ionization state are not responsible for the low storage stability. Possible depletion or rearrangement of water molecules around the active site, or small structural perturbations around the active site or movements of counter ions Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 130
-
 sec-phenethyl alcohol after incubation in 1,4-dioxane, co-lyophilization with methyl-beta-cyclodextrin Bacillus licheniformis
3.4.21.62 319
-
 sec-phenethyl alcohol after a 4-day incubation period in 1,4-dioxane, co-lyophilization with methyl-beta-cyclodextrin Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus licheniformis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 sec-phenethyl alcohol + vinyl butyrate
-
 Bacillus licheniformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 subtilisin C.
-
 Bacillus licheniformis
3.4.21.62 subtilisin Carlsberg
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.62 6.5 8.5
-
 Bacillus licheniformis