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Literature summary extracted from

  • Haskins, N.; Panglao, M.; Qu, Q.; Majumdar, H.; Cabrera-Luque, J.; Morizono, H.; Tuchman, M.; Caldovic, L.
    Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapods (2008), BMC Biochem., 9, 24.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.1 arginine
-
 Mus musculus
2.3.1.1 arginine
-
 Xenopus laevis
2.3.1.1 arginine
-
 Xenopus tropicalis
2.3.1.35 L-arginine
-
 Mus musculus

Application

EC Number Application Comment Organism
2.3.1.1 additional information N-acetylglutamate synthase is a molecular marker for evolution of tetrapods Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Mus musculus
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Escherichia coli
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Arabidopsis thaliana
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Xenopus laevis
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Xanthomonas campestris
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Danio rerio
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Takifugu rubripes
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Xenopus tropicalis
2.3.1.1 cloned in Escherichia coli as a His-tagged fusion protein Maricaulis maris
2.3.1.1 phylogenetic analysis Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.1 E280A specific activity (micromol/min/mg): 26.93, 26.68 (+ 1mM arginine), arginine inhibition is abolished Xanthomonas campestris
2.3.1.1 E280A specific activity (micromol/min/mg): 26.93, 26.68 (+1mM arginine), arginine inhibition is abolished Xanthomonas campestris
2.3.1.1 E354A specific activity (micromol/min/mg): 15.47, 15.28 (+ 1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 E354A specific activity (micromol/min/mg): 15.47, 15.28 (+1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 F121C specific activity (micromol/min/mg): 12.22, 13.08 (+ 1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 F121C specific activity (micromol/min/mg): 12.22, 13.08 (+1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 F35C specific activity (micromol/min/mg): 9.66, 4.31 (+ 1mM arginine), only partial inhibition by arginine Xanthomonas campestris
2.3.1.1 F35C specific activity (micromol/min/mg): 9.66, 4.31 (+1mM arginine), only partial inhibition by arginine Xanthomonas campestris
2.3.1.1 G286P specific activity (micromol/min/mg): 3.51, 3.39 (+ 1mM arginine), no inhibition by arginine Xanthomonas campestris
2.3.1.1 G286P specific activity (micromol/min/mg): 3.51, 3.39 (+1mM arginine), no inhibition by arginine Xanthomonas campestris
2.3.1.1 G288S specific activity (micromol/min/mg): 46.04, 42.55 (+ 1mM arginine), no inhibition by arginine Xanthomonas campestris
2.3.1.1 G288S specific activity (micromol/min/mg): 46.04, 42.55 (+1mM arginine), no inhibition by arginine Xanthomonas campestris
2.3.1.1 G360P specific activity (micromol/min/mg): 13.00, 12.57 (+ 1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 G360P specific activity (micromol/min/mg): 13.00, 12.57 (+1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 G362S specific activity (micromol/min/mg): 3.22, 3.19 (+ 1mM arginine), arginine activation is abolished Mus musculus
2.3.1.1 G362S specific activity (micromol/min/mg): 3.22, 3.19 (+1mM arginine), arginine activation is abolished Mus musculus
2.3.1.35 E280A change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity Xanthomonas campestris
2.3.1.35 E354A change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation Mus musculus
2.3.1.35 F121C change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation Mus musculus
2.3.1.35 F35C change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation leads to partial inhibition of both synthase and kinase activities by arginine and decrease in synthase activity Xanthomonas campestris
2.3.1.35 G286P change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity Xanthomonas campestris
2.3.1.35 G288S change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine inhibition and decreases synthase activity Xanthomonas campestris
2.3.1.35 G360P change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation Mus musculus
2.3.1.35 G362S change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.1 arginine
-
 Arabidopsis thaliana
2.3.1.1 arginine
-
 Danio rerio
2.3.1.1 arginine
-
 Escherichia coli
2.3.1.1 arginine
-
 Maricaulis maris
2.3.1.1 arginine the arginine binding site is localized in the kinase domain. Arginine is an allosteric inhibitor of microbial NAGS as part of feedback regulation of arginine biosynthesis, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS Pseudomonas aeruginosa
2.3.1.1 arginine
-
 Takifugu rubripes
2.3.1.1 arginine
-
 Xanthomonas campestris
2.3.1.35 arginine
-
 Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate Pseudomonas aeruginosa NAGS is the first enzyme of arginine biosynthesis, microbial arginine biosynthesis pathway, overview. Arginine is an allosteric inhibitor of microbial NAGS, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS CoA + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.1 Arabidopsis thaliana
-
-
-
2.3.1.1 Danio rerio
-
-
-
2.3.1.1 Escherichia coli
-
-
-
2.3.1.1 Maricaulis maris
-
-
-
2.3.1.1 Mus musculus
-
-
-
2.3.1.1 Pseudomonas aeruginosa P22567
-
-
2.3.1.1 Takifugu rubripes
-
-
-
2.3.1.1 Xanthomonas campestris
-
-
-
2.3.1.1 Xenopus laevis
-
-
-
2.3.1.1 Xenopus tropicalis
-
-
-
2.3.1.35 Mus musculus
-
-
-
2.3.1.35 Xanthomonas campestris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.1 using Ni-NTA chromatography Mus musculus
2.3.1.1 using Ni-NTA chromatography Escherichia coli
2.3.1.1 using Ni-NTA chromatography Arabidopsis thaliana
2.3.1.1 using Ni-NTA chromatography Xenopus laevis
2.3.1.1 using Ni-NTA chromatography Xanthomonas campestris
2.3.1.1 using Ni-NTA chromatography Danio rerio
2.3.1.1 using Ni-NTA chromatography Takifugu rubripes
2.3.1.1 using Ni-NTA chromatography Xenopus tropicalis
2.3.1.1 using Ni-NTA chromatography Maricaulis maris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.1 additional information
-
 no activity detectable upon addition of 1 mM arginine Arabidopsis thaliana
2.3.1.1 additional information
-
 no activity detectable upon addition of 1 mM arginine Xanthomonas campestris
2.3.1.1 0.098
-
 wild-type Xenopus tropicalis
2.3.1.1 0.136
-
 wild-type + 1 mM arginine Xenopus tropicalis
2.3.1.1 0.438
-
 wild-type Xenopus laevis
2.3.1.1 0.528
-
 wild-type + 1 mM arginine Xenopus laevis
2.3.1.1 0.572
-
 wild-type + 1mM arginine Maricaulis maris
2.3.1.1 1.84
-
 wild-type + 1mM arginine Escherichia coli
2.3.1.1 3.19
-
 mutant G362S + 1 mM arginine Mus musculus
2.3.1.1 3.22
-
 mutant G362S Mus musculus
2.3.1.1 3.39
-
 mutant G286P + 1 mM arginine Xanthomonas campestris
2.3.1.1 3.39
-
 mutant G286P + 1mM arginine Xanthomonas campestris
2.3.1.1 3.51
-
 mutant G286P Xanthomonas campestris
2.3.1.1 4.31
-
 mutant F35C + 1 mM arginine Xanthomonas campestris
2.3.1.1 4.31
-
 mutant F35C + 1mM arginine Xanthomonas campestris
2.3.1.1 5.475
-
 wild-type + 1 mM arginine Danio rerio
2.3.1.1 5.85
-
 wild-type Escherichia coli
2.3.1.1 9.11
-
 wild-type Arabidopsis thaliana
2.3.1.1 9.66
-
 mutant F35C Xanthomonas campestris
2.3.1.1 10.64
-
 wild-type Danio rerio
2.3.1.1 12.22
-
 mutant F121C Mus musculus
2.3.1.1 12.27
-
 wild-type Mus musculus
2.3.1.1 12.57
-
 mutant G360P + 1 mM arginine Mus musculus
2.3.1.1 12.57
-
 mutant G360P + 1mM arginine Mus musculus
2.3.1.1 13
-
 mutant G360P Mus musculus
2.3.1.1 13.08
-
 mutant F121C + 1 mM arginine Mus musculus
2.3.1.1 15.28
-
 mutant E354A + 1 mM arginine Mus musculus
2.3.1.1 15.47
-
 mutant E354A Mus musculus
2.3.1.1 15.89
-
 wild-type + 1 mM arginine Takifugu rubripes
2.3.1.1 20.88
-
 wild-type Takifugu rubripes
2.3.1.1 25.56
-
 wild-type + 1 mM arginine Mus musculus
2.3.1.1 26.68
-
 mutant E280A + 1 mM arginine Xanthomonas campestris
2.3.1.1 26.68
-
 mutant E280A + 1mM arginine Xanthomonas campestris
2.3.1.1 26.93
-
 mutant E280A Xanthomonas campestris
2.3.1.1 42.55
-
 mutant G288S + 1 mM arginine Xanthomonas campestris
2.3.1.1 42.55
-
 mutant G288S + 1mM arginine Xanthomonas campestris
2.3.1.1 46.04
-
 mutant G288S Xanthomonas campestris
2.3.1.1 62
-
 wild-type Maricaulis maris
2.3.1.1 71
-
 wild-type Xanthomonas campestris
2.3.1.1 111.7
-
 wild-type Xanthomonas campestris
2.3.1.35 3.2
-
 mutant G362S Mus musculus
2.3.1.35 3.2
-
 mutant G362S, presence of 1 mM L-arginine Mus musculus
2.3.1.35 3.4
-
 mutant G286P, presence of 1 mM L-arginine Xanthomonas campestris
2.3.1.35 3.5
-
 mutant G286P Xanthomonas campestris
2.3.1.35 4.3
-
 mutant F35C, presence of 1 mM L-arginine Xanthomonas campestris
2.3.1.35 9.7
-
 mutant F35C Xanthomonas campestris
2.3.1.35 12.2
-
 mutant F121C Mus musculus
2.3.1.35 12.3
-
 wild-type Mus musculus
2.3.1.35 12.3
-
 wild-type, presence of 1 mM L-arginine Mus musculus
2.3.1.35 12.6
-
 mutant G360P, presence of 1 mM L-arginine Mus musculus
2.3.1.35 13
-
 mutant G360P Mus musculus
2.3.1.35 13.1
-
 mutant E354A, presence of 1 mM L-arginine Mus musculus
2.3.1.35 15.3
-
 mutant F121C, presence of 1 mM L-arginine Mus musculus
2.3.1.35 15.5
-
 mutant E354A Mus musculus
2.3.1.35 26.7
-
 mutant E280A, presence of 1 mM L-arginine Xanthomonas campestris
2.3.1.35 26.9
-
 mutant E280A Xanthomonas campestris
2.3.1.35 42.6
-
 mutant G288S, presence of 1 mM L-arginine Xanthomonas campestris
2.3.1.35 46
-
 mutant G288S Xanthomonas campestris
2.3.1.35 71
-
 wild-type Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate
-
 Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate NAGS is the first enzyme of arginine biosynthesis, microbial arginine biosynthesis pathway, overview. Arginine is an allosteric inhibitor of microbial NAGS, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Mus musculus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Xanthomonas campestris L-ornithine + N-acetyl-L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.1 N-acetylglutamate synthase
-
 Mus musculus
2.3.1.1 N-acetylglutamate synthase
-
 Escherichia coli
2.3.1.1 N-acetylglutamate synthase
-
 Arabidopsis thaliana
2.3.1.1 N-acetylglutamate synthase
-
 Xenopus laevis
2.3.1.1 N-acetylglutamate synthase
-
 Xanthomonas campestris
2.3.1.1 N-acetylglutamate synthase
-
 Danio rerio
2.3.1.1 N-acetylglutamate synthase
-
 Takifugu rubripes
2.3.1.1 N-acetylglutamate synthase
-
 Xenopus tropicalis
2.3.1.1 N-acetylglutamate synthase
-
 Maricaulis maris
2.3.1.1 N-acetylglutamate synthase
-
 Pseudomonas aeruginosa
2.3.1.1 NAGS
-
 Mus musculus
2.3.1.1 NAGS
-
 Escherichia coli
2.3.1.1 NAGS
-
 Arabidopsis thaliana
2.3.1.1 NAGS
-
 Xenopus laevis
2.3.1.1 NAGS
-
 Xanthomonas campestris
2.3.1.1 NAGS
-
 Danio rerio
2.3.1.1 NAGS
-
 Takifugu rubripes
2.3.1.1 NAGS
-
 Xenopus tropicalis
2.3.1.1 NAGS
-
 Maricaulis maris
2.3.1.1 NAGS
-
 Pseudomonas aeruginosa
2.3.1.1 XcNAGS
-
 Xanthomonas campestris

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.1 acetyl-CoA
-
 Pseudomonas aeruginosa