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Literature summary extracted from

  • Ogino, H.; Nakayama, H.; China, H.; Kawata, T.; Doukyu, N.; Yasuda, M.
    Characterization of recombinant glyoxylate reductase from thermophile Thermus thermophilus HB27 (2008), Biotechnol. Prog., 24, 321-325.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.79 expressed in Escherichia coli Top10 as His-tag fusion protein Thermus thermophilus HB27
1.1.1.79 expression of the Myc-His9-tagged enzyme in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 additional information no activity at more than 500 mM NaCl, 50% activity in the presence of 900 mM sodium phosphate Thermus thermophilus HB27
1.1.1.79 NaCl complete inhibition at 0.5 M Thermus thermophilus
1.1.1.79 Sodium phosphate 50% inhibition at 900 mM Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.004
-
 NADPH pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme Thermus thermophilus
1.1.1.79 0.04
-
 NADPH with glyoxylate as substrate, pH 6.7, 45°C Thermus thermophilus HB27
1.1.1.79 0.076
-
 NADH pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme Thermus thermophilus
1.1.1.79 0.076
-
 NADH with glyoxylate as substrate, pH 6.7, 45°C Thermus thermophilus HB27
1.1.1.79 0.5
-
 glyoxylate pH 6.7, 45°C, cofactor NADPH, purified recombinant enzyme Thermus thermophilus
1.1.1.79 0.5
-
 glyoxylate with as NADPH as cofactor, pH 6.7, 45°C Thermus thermophilus HB27
1.1.1.79 1.2
-
 glyoxylate pH 6.7, 45°C, cofactor NADH, purified recombinant enzyme Thermus thermophilus
1.1.1.79 1.2
-
 glyoxylate with as NADH as cofactor, pH 6.7, 45°C Thermus thermophilus HB27

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.79 36000
-
 2 * 36000, SDS-PAGE, native mass by gel filtration (native mass not given in the reference) Thermus thermophilus HB27

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H Thermus thermophilus
-
 glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
 glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ Thermus thermophilus HB27
-
 glycolate + NADP+
-
 ir

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.79 1,5-pentanediol 25%, half life reduced by approximately 70% Thermus thermophilus HB27
1.1.1.79 diethylene glycol half-life in the presence of 25% diethylene glycol significantly longer than that in the absence of an organic solvent Thermus thermophilus HB27
1.1.1.79 dimethyl sulfoxide half-life in the presence of 25% dimethyl sulfoxide significantly longer than that in the absence of an organic solvent Thermus thermophilus HB27
1.1.1.79 Ethanol 25%, half life reduced by approximately 70% Thermus thermophilus HB27
1.1.1.79 Glycerol 25%, unstable Thermus thermophilus HB27
1.1.1.79 n-decane 25%, unstable Thermus thermophilus HB27
1.1.1.79 n-hexane 25%, unstable Thermus thermophilus HB27
1.1.1.79 n-octane 25%, unstable Thermus thermophilus HB27
1.1.1.79 p-xylene 25%, unstable Thermus thermophilus HB27
1.1.1.79 toluene 25%, unstable Thermus thermophilus HB27

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Thermus thermophilus
-
-
-
1.1.1.79 Thermus thermophilus HB27
-
 NBRC code 101085
-
1.1.1.79 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 recombinant C-terminally His9-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Thermus thermophilus
1.1.1.79 recombinant protein from Escherichia coli using His-tag Thermus thermophilus HB27

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H
-
 Thermus thermophilus glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NADH + H+
-
 Thermus thermophilus glycolate + NAD+
-
 r
1.1.1.79 glyoxylate + NADH + H+
-
 Thermus thermophilus HB27 glycolate + NAD+
-
 ir
1.1.1.79 glyoxylate + NADH + H+
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 glycolate + NAD+
-
 r
1.1.1.79 glyoxylate + NADPH + H+
-
 Thermus thermophilus HB27 glycolate + NADP+
-
 ir
1.1.1.79 additional information phenylpyruvate, pyruvate, methylglyoxal, malate, and oxaloacetate not suitable as substrate Thermus thermophilus HB27 ?
-
 ?
1.1.1.79 additional information substrate specificity, several 2-oxo compounds including phenylpyruvate, pyruvate, methylglyoxal, and oxaloacetate act as inert electron acceptors, as do glycolate and malate, overview Thermus thermophilus ?
-
 ?
1.1.1.79 additional information substrate specificity, several 2-oxo compounds including phenylpyruvate, pyruvate, methylglyoxal, and oxaloacetate act as inert electron acceptors, as do glycolate and malate, overview Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.79 dimer 2 * 36000, SDS-PAGE, native mass by gel filtration (native mass not given in the reference) Thermus thermophilus HB27

Synonyms

EC Number Synonyms Comment Organism
1.1.1.79 More cf. 1.1.1.26 Thermus thermophilus
1.1.1.79 TthGR1 belongs to the D-2-hydroxyacid dehydrogenase family, enzyme used NADPH and NADH (EC 1.1.1.26 activity) as cofactor Thermus thermophilus HB27

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.79 45
-
 recombinant enzyme Thermus thermophilus
1.1.1.79 45
-
 recombinant protein Thermus thermophilus HB27

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.79 70
-
 10 min, recombinant enzyme, stable Thermus thermophilus
1.1.1.79 70
-
 stable up to 70°C Thermus thermophilus HB27
1.1.1.79 80
-
 complete loss of activity within 10 min when incubates above 80°C Thermus thermophilus HB27
1.1.1.79 82
-
 melting point Thermus thermophilus HB27

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.79 22000
-
 NADH pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme Thermus thermophilus
1.1.1.79 30000
-
 NADPH pH 6.7, 45°C, substrate glyoxylate, purified recombinant enzyme Thermus thermophilus
1.1.1.79 65000
-
 glyoxylate pH 6.7, 45°C, cofactor NADPH, purified recombinant enzyme Thermus thermophilus
1.1.1.79 76000
-
 glyoxylate pH 6.7, 45°C, cofactor NADH, purified recombinant enzyme Thermus thermophilus
1.1.1.79 220000
-
 NADH with glyoxylate as substrate, pH 6.7, 45°C Thermus thermophilus HB27
1.1.1.79 300000
-
 NADPH with glyoxylate as substrate, pH 6.7, 45°C Thermus thermophilus HB27
1.1.1.79 650000
-
 glyoxylate with as NADPH as cofactor, pH 6.7, 45°C Thermus thermophilus HB27
1.1.1.79 760000
-
 glyoxylate with as NADH as cofactor, pH 6.7, 45°C Thermus thermophilus HB27

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 6.7
-
 recombinant enzyme Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.79 6.7
-
 recombinant protein Thermus thermophilus HB27

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NADH less active than NADPH Thermus thermophilus
1.1.1.79 NADH yields higher Km but similar turnover than NADPH Thermus thermophilus HB27
1.1.1.79 NADPH is the preferred cofactor Thermus thermophilus
1.1.1.79 NADPH preferred cofactor, yields lower Km but similar turnover than NADH Thermus thermophilus HB27