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Literature summary extracted from

  • Hong, E.S.; Kwon, O.Y.; Ryu, K.
    Strong substrate-stabilizing effect of a water-miscible ionic liquid [BMIM][BF(4)] in the catalysis of horseradish peroxidase (2008), Biotechnol. Lett., 30, 529-533.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 1-butyl-3-methylimidazolium tetrafluoroborate weak, non-competitive inhibitor Armoracia rusticana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 2.8
-
 guaiacol in the absence of 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 4.1
-
 guaiacol in the presence of 5% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 6.8
-
 guaiacol in the presence of 10% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 7.8
-
 guaiacol in the presence of 15% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 15.1
-
 guaiacol in the presence of 20% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 22.5
-
 guaiacol in the presence of 25% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Armoracia rusticana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 guaiacol + H2O2
-
 Armoracia rusticana tetraguaiacol + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 horseradish peroxidase
-
 Armoracia rusticana
1.11.1.7 HRP
-
 Armoracia rusticana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 8.7
-
 guaiacol in the presence of 25% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 9.3
-
 guaiacol in the presence of 20% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 10.6
-
 guaiacol in the presence of 15% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 10.9
-
 guaiacol in the presence of 10% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 12.2
-
 guaiacol in the absence of 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
1.11.1.7 12.4
-
 guaiacol in the presence of 5% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.7 2900
-
 1-butyl-3-methylimidazolium tetrafluoroborate at 25°C Armoracia rusticana