Any feedback?
Literature summary extracted from
- Tyrosinase activity in ionic liquids (2008), Biotechnol. Lett., 30, 153-158.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.3 | - |
4-methylcatechol | in aqueous solution | Agaricus bisporus |  |
| 1.14.18.1 | 0.5 | - |
4-methylcatechol | in CHCl3 | Agaricus bisporus | |
| 1.14.18.1 | 33 | - |
4-methylcatechol | in 1-butyl-3-methylimidazolium hexafluorophosphate, much higher than those obtained in CHCl3 and aqueous solution | Agaricus bisporus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Agaricus bisporus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | enzyme is extracted into 100 ml phosphate buffer (50 mM, pH 6.0) and crudely purified from fresh mushrooms (50 g) and then adsorbed onto celite (2 g) and dried in a vacuum oven | Agaricus bisporus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
activity of mushroom tyrosinase is studied in three ionic liquids, 1-butyl-3-methylimidazolium hexafluorophosphate (1-butyl-3-methylimidazolium hexafluorophosphate), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]) and 1-butyl-3-methylimidazolium methylsulfate ([BMIm] [MeSO4]), and is compared to that in chloroform. Enzyme follows in ionic liquids basically the same catalytic mechanism as in water | Agaricus bisporus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 4-methylcatechol + 1/2 O2 | oxidation of the substrate with NaIO4 in CHCl3, and [P]CHCl3 | Agaricus bisporus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | polyphenol oxidase | - |
Agaricus bisporus |
| 1.14.18.1 | tyrosinase | - |
Agaricus bisporus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 20 | - |
optimal reaction temperature for the enzyme in chloroform | Agaricus bisporus |
| 1.14.18.1 | 45 | - |
optimal reaction temperature for the enzyme in aqueous solution | Agaricus bisporus |
| 1.14.18.1 | 50 | - |
optimal reaction temperature of the enzyme in 1-butyl-3-methylimidazolium hexafluorophosphate should be higher than 50°C, which is higher than the one for the enzyme in chloroform (20°C) and in aqueous solution (45°C) | Agaricus bisporus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 20 | 50 | - |
Agaricus bisporus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
