Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nam, S.H.; Ko, E.A.; Jang, S.S.; Kim, d.o..W.; Kim, S.Y.; Hwang, D.S.; Kim, D.
    Maximization of dextransucrase activity expressed in E. coli by mutation and its functional characterization (2008), Biotechnol. Lett., 30, 135-143.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene DSRB742, expression of wild-type and DRN1-DRN4 mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 F196S site-directed mutagenesis, the mutant DRN1 shows a higher expression level in Escherichia coli and increased activity compared to DSRB742 Leuconostoc mesenteroides
2.4.1.5 K395T site-directed mutagenesis, the mutant DRN3 shows a higher expression level in Escherichia coli and increased activity compared to DSRB742 Leuconostoc mesenteroides
2.4.1.5 P980T site-directed mutagenesis, the mutant DRN4 shows a lower expression level in Escherichia coli compared to DSRB742 Leuconostoc mesenteroides
2.4.1.5 Y346N site-directed mutagenesis, the mutant DRN2 shows a lower expression level in Escherichia coli compared to DSRB742 Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 18
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN1 Leuconostoc mesenteroides
2.4.1.5 48
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN3 Leuconostoc mesenteroides
2.4.1.5 79
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN4 Leuconostoc mesenteroides
2.4.1.5 83
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN2 Leuconostoc mesenteroides
2.4.1.5 88
-
 sucrose pH 5.2, 28°C, recombinant wild-type enzyme Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 gene DSRB742
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 recombinant wild-type and DRN1-DRN4 mutant enzymes from Escherichia coli by nickel affinity chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 0.012
-
 purified recombinant mutant DRN4 Leuconostoc mesenteroides
2.4.1.5 0.027
-
 purified recombinant mutant DRN2 Leuconostoc mesenteroides
2.4.1.5 0.037
-
 purified recombinant wild-type DSRB742 Leuconostoc mesenteroides
2.4.1.5 0.185
-
 purified recombinant mutant DRN3 Leuconostoc mesenteroides
2.4.1.5 0.287
-
 purified recombinant mutant DRN1 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + gentobiose glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview Leuconostoc mesenteroides ?
-
 ?
2.4.1.5 sucrose + maltose glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview Leuconostoc mesenteroides ?
-
 ?
2.4.1.5 sucrose + salicin glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview Leuconostoc mesenteroides ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DSRB742
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 28
-
 assay at Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.5 0.4
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN4 Leuconostoc mesenteroides
2.4.1.5 0.6
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN2 Leuconostoc mesenteroides
2.4.1.5 1.8
-
 sucrose pH 5.2, 28°C, recombinant wild-type enzyme Leuconostoc mesenteroides
2.4.1.5 2.4
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN1 Leuconostoc mesenteroides
2.4.1.5 2.8
-
 sucrose pH 5.2, 28°C, recombinant mutant DRN3 Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.2
-
 assay at Leuconostoc mesenteroides