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Literature summary extracted from

  • Lee, J.; Oh, D.; Song, H.; Kim, I.
    Ca2+ and Cu2+ supplementation increases mannitol production by Candida magnoliae (2007), Biotechnol. Lett., 29, 291-294.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.138 synthesis the enzyme of strain strain HH-01, KCCM-10252, is useful in production of D-mannitol Starmerella magnoliae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.138 Cu2+ increases the intracellular activity of mannitol dehydrogenase 1.6fold Starmerella magnoliae
1.1.1.138 additional information Ca2+ reduces the intracellular mannitol concetration by 30% probably by altering the permeability of cells for mannito Starmerella magnoliae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-mannitol + NADP+ Starmerella magnoliae
-
 D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+ Starmerella magnoliae HH-01
-
 D-fructose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Starmerella magnoliae
-
-
-
1.1.1.138 Starmerella magnoliae HH-01
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 additional information optimization of culture conditions, overview, supplementation with CaCl2 x 2 H2O at 50 mg/l or CuSO4+ x 5 H2O at 10 mg/l improves mannitol production by Candida magnoliae by 14.5 and 18.6% to 25 and 32 g/l, respectively Starmerella magnoliae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 0.5 0.9 crude enzyme extract, optimization of culture conditions, overview Starmerella magnoliae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-mannitol + NADP+
-
 Starmerella magnoliae D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Starmerella magnoliae HH-01 D-fructose + NADPH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7.8
-
 assay at Starmerella magnoliae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+
-
 Starmerella magnoliae
1.1.1.138 NADPH
-
 Starmerella magnoliae