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Literature summary extracted from

  • Li, D.; Cheng, S.; Wei, D.; Ren, Y.; Zhang, D.
    Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium (2007), Biotechnol. Lett., 29, 1825-1830.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis penicillin G acylase-catalyzed acylation of beta-phenylalanine with phenylacetamide as an acyl donor is efficient with a high preferential enantioselectivity for (R)-beta-phenylalanine. Both (S)-beta-phenylalanine, at 98% ee, and (R)-beta-phenylalanine, at 99% ee, can be produced using simple separation procedures Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 (R)-beta-phenylalanine + phenylacetamide the acylation reaction is highly preferential for the acylation of (R)-beta-phenylalanine Escherichia coli N-phenylacetyl-(R)-beta-phenylalanine + ?
-
 ?
3.5.1.11 penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanate + phenylacetic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin G acylase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 10
-
 acylation of beta-phenylalanine Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.11 8 11 pH 8: about 50% of maximal activity, pH 11: about 60% of maximal activity Escherichia coli