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Literature summary extracted from
- Catalytic properties of beta -cyclodextrin glucanotransferase from alkalophilic Bacillus sp. BL-12 and intermolecular transglycosylation of stevioside (2007), Biotechnol. Bioprocess Eng., 12, 207-212.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.19 | 15.54 | - |
beta-cyclodextrin | pH 8.5, 50°C | Bacillus sp. (in: Bacteria) |  |
| 2.4.1.19 | 15.54 | - |
soluble starch | pH 8.5, 50°C | Bacillus sp. (in: Bacteria) | |
| 2.4.1.19 | 15.54 | - |
4-nitrophenyl-beta-D-glucopyranose | pH 8.5, 50°C | Bacillus sp. (in: Bacteria) | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.19 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 90000 | - |
x * 90000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.19 | Bacillus sp. (in: Bacteria) | - |
- |
- |
| 2.4.1.19 | Bacillus sp. (in: Bacteria) BL-12 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | native enzyme 11.4fold from strain BL-12 by ultrafiltration and beta-cyclodextrin polymer affinity chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 205 | - |
purified enzyme | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.19 | beta-cyclodextrin + 4-nitrophenyl-beta-D-glucopyranose | beta-cyclodextrin as a glycosyl donor and 4-nitrophenyl-beta-D-glucopyranose as a glycosyl acceptor | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 2.4.1.19 | beta-cyclodextrin + 4-nitrophenyl-beta-D-glucopyranose | beta-cyclodextrin as a glycosyl donor and 4-nitrophenyl-beta-D-glucopyranose as a glycosyl acceptor | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
| 2.4.1.19 | additional information | glycosyl donor specificity for intermolecular transglycosylation of stevioside, overview, cyclization reaction with soluble starch as substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 2.4.1.19 | additional information | glycosyl donor specificity for intermolecular transglycosylation of stevioside, overview, cyclization reaction with soluble starch as substrate | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
| 2.4.1.19 | stevioside + maltodextrin | the enzyme from strain BL-12 is more suitable for transglycosylation than the cyclization reaction, and is specific for the intermolecular transglycosylation of stevioside with maltodextrin as the most suitable glycosyl donor | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 2.4.1.19 | stevioside + maltodextrin | the enzyme from strain BL-12 is more suitable for transglycosylation than the cyclization reaction, and is specific for the intermolecular transglycosylation of stevioside with maltodextrin as the most suitable glycosyl donor | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
| 2.4.1.19 | stevioside + starch | soluble, extrusion, or potato starch | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 2.4.1.19 | stevioside + starch | soluble, extrusion, or potato starch | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | ? | x * 90000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | beta-CGTase | - |
Bacillus sp. (in: Bacteria) |
| 2.4.1.19 | beta-cyclodextrin glucanotransferase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 20 | 70 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 40 | - |
purified native enzyme, thermal stability at 40°C is elevated 10fold in the presence of 1% maltodextrin | Bacillus sp. (in: Bacteria) |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 9 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 5 | 11 | - |
Bacillus sp. (in: Bacteria) |
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