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Literature summary extracted from

  • Wu, S.; Fogiel, A.J.; Petrillo, K.L.; Jackson, R.E.; Parker, K.N.; DiCosimo, R.; Ben-Bassat, A.; OKeefe, D.P.; Payne, M.S.
    Protein engineering of nitrilase for chemoenzymatic production of glycolic acid (2007), Biotechnol. Bioeng., 99, 717-720.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 F168I/L201E 4.6fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 F168K 3.1fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 F168M 3fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 F168T 1.7fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 F168T/L201Q 3.1fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 F168V 4.1fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 F168V/L201N 15.3fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201A 1.9fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201G 1.5fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201H 2.7fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201K 3.5fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201N 5.5fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201Q 4.9fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201S 2.2fold increased specific activity compared to the wild type enzyme Acidovorax facilis
3.5.5.1 L201T 2fold increased specific activity compared to the wild type enzyme Acidovorax facilis

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Acidovorax facilis
-
-
-
3.5.5.1 Acidovorax facilis 72W
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 benzonitrile + 2 H2O
-
 Acidovorax facilis benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Acidovorax facilis 72W benzoic acid + NH3
-
 ?
3.5.5.1 glycolonitrile + H2O
-
 Acidovorax facilis ammonium glycolate + ?
-
 ?
3.5.5.1 glycolonitrile + H2O
-
 Acidovorax facilis 72W ammonium glycolate + ?
-
 ?