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Literature summary extracted from

  • Mansfeld, J.; Ulbrich-Hofmann, R.
    The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents (2007), Biotechnol. Bioeng., 97, 672-679.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.28 G8C/N60C double mutant temperature optimum at 80°C, increased thermal stability Geobacillus stearothermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.24.28 2-propanol 48 h, 37°C, 50% residual activity with 44.5% (v/v) and 65.5% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
3.4.24.28 Acetone 48 h, 37°C, 50% residual activity with 30.0% (v/v) and 50.1% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
3.4.24.28 acetonitrile 48 h, 37°C, 50% residual activity with 30.6% (v/v) and 51.6% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
3.4.24.28 dimethylformamide 48 h, 37°C, 50% residual activity with 29.8% (v/v) and 45.8% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
3.4.24.28 dioxane 48 h, 37°C, 50% residual activity with 32.1% (v/v) and 47.9% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
3.4.24.28 DMSO 48 h, 37°C, 50% residual activity with 32.9% (v/v) and 48.7% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
3.4.24.28 Methanol 48 h, 37°C, 50% residual activity with 56.6% (v/v) and 86.4% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.28 Geobacillus stearothermophilus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.28 78.5
-
Boilysin at 37°C with casein as substrate Geobacillus stearothermophilus
3.4.24.28 81.5
-
pseudo wild type at 37°C with casein as substrate Geobacillus stearothermophilus
3.4.24.28 81.9
-
wild type enzyme at 37°C with casein as substrate Geobacillus stearothermophilus
3.4.24.28 82.4
-
double mutant G8C/N60C at 37°C with casein as substrate Geobacillus stearothermophilus
3.4.24.28 242
-
wild type enzyme at temperature optimum 75°C Geobacillus stearothermophilus
3.4.24.28 273
-
pseudo wild type at temperature optimum 75°C Geobacillus stearothermophilus
3.4.24.28 375
-
double mutant G8C/N60C at temperature optimum 80°C Geobacillus stearothermophilus
3.4.24.28 608
-
boilysin at temperature optimum 95°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.28 3-(2-furylacryloyl)-glycyl-L-leucinamide + H2O
-
Geobacillus stearothermophilus ?
-
?
3.4.24.28 casein + H2O
-
Geobacillus stearothermophilus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.28 75
-
wild type enzyme Geobacillus stearothermophilus
3.4.24.28 75
-
pseudo wild type Geobacillus stearothermophilus
3.4.24.28 80
-
double mutant G8C/N60C Geobacillus stearothermophilus
3.4.24.28 95
-
boilysin Geobacillus stearothermophilus