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Literature summary extracted from

  • Bhambi, M.; Pundir, C.S.
    Immobilization of grain-sorghum (Sorghum bicolor, var. CSH-14) leaf oxalate oxidase on to a modified mica chip and its application in the determination of urinary oxalate (2007), Biotechnol. Appl. Biochem., 47, 125-129.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.3.4 the immobilized enzyme on modified mica chip retains 65.35% of the specific activity of free enzyme Sorghum bicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 ascorbate ascorbate causes 80% inhibition in the activity of immobilized enzyme Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.53
-
 oxalic acid free enzyme Sorghum bicolor
1.2.3.4 0.7
-
 oxalic acid immobilized enzyme Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Sorghum bicolor
-
 variant CSH-14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4 ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Sephadex G-100 gel filtration Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 leaf
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Sorghum bicolor 2 CO2 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.3.4 OXO
-
 Sorghum bicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.4 40
-
 free enzyme Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 5
-
 free enzyme Sorghum bicolor
1.2.3.4 6.5
-
 the immobilized enzyme shows maximum activity at pH 6.5 when incubated at 40°C for 15 min Sorghum bicolor