BRENDA - Enzyme Database

Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium

Liao, S.; Poonpairoj, P.; Ko, K.; Takatuska, Y.; Yamaguchi, Y.; Abe, N.; Kaneko, J.; Kamio, Y.; Biosci. Biotechnol. Biochem. 72, 445-455 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.53
expressed in Escherichia coli; expression in Escherichia coli strain DE3
Selenomonas ruminantium
3.5.3.12
expression in Escherichia coli strain DE3
Selenomonas ruminantium
4.1.1.19
expression in Escherichia coli strain DE3
Selenomonas ruminantium
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.53
C153A
inactive mutant enzyme; mutant without enzyme activity
Selenomonas ruminantium
3.5.1.53
E160A
inactive mutant enzyme; mutant without enzyme activity
Selenomonas ruminantium
3.5.3.12
C361A
20% enzyme activity of wild-type enzyme
Selenomonas ruminantium
General Stability
EC Number
General Stability
Organism
3.5.1.53
in 50 mM PIPES buffer at pH 6.5 stable for 30 min up to 70°C
Selenomonas ruminantium
4.1.1.19
at 50°C in absence of PALP lost of 90% enzyme activity within 10 min, no enzyme activity after heating at 70°C for 60 min in presence of PALP
Selenomonas ruminantium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.53
Co2+
; strong inhibitor
Selenomonas ruminantium
3.5.1.53
Fe2+
; strong inhibitor
Selenomonas ruminantium
3.5.1.53
Hg2+
; strong inhibitor
Selenomonas ruminantium
3.5.1.53
iodoacetamide
; strong inhibitor
Selenomonas ruminantium
3.5.1.53
Mn2+
; strong inhibitor
Selenomonas ruminantium
3.5.1.53
Zn2+
; strong inhibitor
Selenomonas ruminantium
3.5.3.12
Al3+
1 mM
Selenomonas ruminantium
3.5.3.12
Co2+
1 mM
Selenomonas ruminantium
3.5.3.12
Cu2+
1 mM
Selenomonas ruminantium
3.5.3.12
Fe2+
1 mM
Selenomonas ruminantium
3.5.3.12
Fe3+
1 mM
Selenomonas ruminantium
3.5.3.12
Hg2+
strong inhibitor
Selenomonas ruminantium
3.5.3.12
iodoacetamide
strong inhibitor
Selenomonas ruminantium
3.5.3.12
Ni2+
1 mM
Selenomonas ruminantium
3.5.3.12
Zn2+
1 mM
Selenomonas ruminantium
4.1.1.19
DL-alpha-difluoromethylarginine
-
Selenomonas ruminantium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.53
0.22
-
N-Carbamoylputrescine
;
Selenomonas ruminantium
3.5.3.12
6.67
-
agmatine
-
Selenomonas ruminantium
4.1.1.19
5.6
-
L-arginine
-
Selenomonas ruminantium
4.1.1.19
50
-
L-lysine
-
Selenomonas ruminantium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.53
32532
-
2 * 32532, calculated from sequence
Selenomonas ruminantium
3.5.1.53
37000
-
2 * 37000, SDS-PAGE; SDS-PAGE
Selenomonas ruminantium
3.5.1.53
80000
-
gel filtration; gel filtration
Selenomonas ruminantium
3.5.3.12
45000
-
gel filtration; SDS-PAGE
Selenomonas ruminantium
4.1.1.19
58000
-
SDS-PAGE
Selenomonas ruminantium
4.1.1.19
120000
-
gel filtration
Selenomonas ruminantium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.53
Selenomonas ruminantium
Q5KR04
-
-
3.5.1.53
Selenomonas ruminantium
-
-
-
3.5.3.12
Selenomonas ruminantium
Q5KR05
-
-
4.1.1.19
Selenomonas ruminantium
Q5NT93
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.53
; recombinant
Selenomonas ruminantium
3.5.3.12
-
Selenomonas ruminantium
4.1.1.19
2024fold after final purification step with MonoQ HR 5/5
Selenomonas ruminantium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.19
0.00738
-
crude extract
Selenomonas ruminantium
4.1.1.19
0.01104
-
heat treatment
Selenomonas ruminantium
4.1.1.19
0.02394
-
after purification with DEAE-Toyopearl 650M
Selenomonas ruminantium
4.1.1.19
0.0381
-
after purification with DEAE-5PW
Selenomonas ruminantium
4.1.1.19
1.026
-
at the semi-final step of purification with HA-1000 column chromatography
Selenomonas ruminantium
4.1.1.19
14.94
-
final step of purification with MonoQ HR 5/5
Selenomonas ruminantium
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.19
4°C, 1 month, no lost of enzyme activity, pH 6.0-7.5
Selenomonas ruminantium
4.1.1.19
50°C, absence of PALP, 10 min, only 10% residual activity
Selenomonas ruminantium
4.1.1.19
70°C, presence of PALP, 60 min, no residual activity
Selenomonas ruminantium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.53
N-carbamoylputrescine + H2O
-
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
3.5.1.53
N-carbamoylputrescine + H2O
10 mM, assay at pH 6.5, 10 min, 40°C
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
25 mM, assay at pH 6.5, 10 min, 40°C
685687
Selenomonas ruminantium
putrescine + ?
-
-
-
?
4.1.1.19
L-arginine
assay at pH 6.5, 10 min, 50°C
685687
Selenomonas ruminantium
agmatine + CO2
-
-
-
?
4.1.1.19
L-lysine
assay at pH 6.5, 10 min, 50°C
685687
Selenomonas ruminantium
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.53
dimer
2 * 32532, calculated from sequence; 2 * 37000, SDS-PAGE
Selenomonas ruminantium
3.5.1.53
homodimer
-
Selenomonas ruminantium
3.5.3.12
monomer
-
Selenomonas ruminantium
4.1.1.19
dimer
-
Selenomonas ruminantium
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.53
45
-
; assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
3.5.3.12
40
-
assay at
Selenomonas ruminantium
4.1.1.19
60
-
-
Selenomonas ruminantium
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.19
40
70
after 10 min incubation at 50°C 4fold higher enzyme activity than at 40°C, after 10 min incubation at 55°C 7fold higher enzyme activity than at 40°C, after 10 min incubation at 60°C 10fold higher enzyme activity than at 40°C, after 10 min incubation at 70°C 1.2fold higher enzyme activity than at 40°C
Selenomonas ruminantium
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.53
70
-
AguB activity is stable at up to 70°C for 30 min in 50 mM PIPES buffer, pH 6.5; up to 70°C, stable for 30 min at pH 6.5, 50 mM PIPES buffer
Selenomonas ruminantium
3.5.3.12
40
-
below 40°C stable for 30 min, pH 6.0-9.0
Selenomonas ruminantium
4.1.1.19
35
-
without pyridoxal 5'-phosphate, 50% redsidual enzyme activity
Selenomonas ruminantium
4.1.1.19
60
-
with pyridoxal 5'-phosphate, 54% residual enzyme activity
Selenomonas ruminantium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.53
7
-
; assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
3.5.3.12
6.5
-
assay at
Selenomonas ruminantium
4.1.1.19
6.5
-
assay at pH 6.5, 10 min, 50°C
Selenomonas ruminantium
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
6.5
9
80% enzyme activity at pH 7.0, 70% enzyme activity at pH 8.0, 50% enzyme activity at pH 9.0
Selenomonas ruminantium
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.53
6
8
; stable
Selenomonas ruminantium
3.5.3.12
6
9
30 min, temperature below 40°C
Selenomonas ruminantium
4.1.1.19
6.5
7.5
more than 80% enzyme activity, decreased enzyme activity at higher pH
Selenomonas ruminantium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.19
pyridoxal 5'-phosphate
-
Selenomonas ruminantium
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.1.19
0.8
-
DL-alpha-difluoromethylarginine
-
Selenomonas ruminantium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.53
expressed in Escherichia coli
Selenomonas ruminantium
3.5.1.53
expression in Escherichia coli strain DE3
Selenomonas ruminantium
3.5.3.12
expression in Escherichia coli strain DE3
Selenomonas ruminantium
4.1.1.19
expression in Escherichia coli strain DE3
Selenomonas ruminantium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.19
pyridoxal 5'-phosphate
-
Selenomonas ruminantium
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.53
C153A
inactive mutant enzyme
Selenomonas ruminantium
3.5.1.53
C153A
mutant without enzyme activity
Selenomonas ruminantium
3.5.1.53
E160A
inactive mutant enzyme
Selenomonas ruminantium
3.5.1.53
E160A
mutant without enzyme activity
Selenomonas ruminantium
3.5.3.12
C361A
20% enzyme activity of wild-type enzyme
Selenomonas ruminantium
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.53
in 50 mM PIPES buffer at pH 6.5 stable for 30 min up to 70°C
Selenomonas ruminantium
4.1.1.19
at 50°C in absence of PALP lost of 90% enzyme activity within 10 min, no enzyme activity after heating at 70°C for 60 min in presence of PALP
Selenomonas ruminantium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.53
Co2+
-
Selenomonas ruminantium
3.5.1.53
Co2+
strong inhibitor
Selenomonas ruminantium
3.5.1.53
Fe2+
-
Selenomonas ruminantium
3.5.1.53
Fe2+
strong inhibitor
Selenomonas ruminantium
3.5.1.53
Hg2+
-
Selenomonas ruminantium
3.5.1.53
Hg2+
strong inhibitor
Selenomonas ruminantium
3.5.1.53
iodoacetamide
-
Selenomonas ruminantium
3.5.1.53
iodoacetamide
strong inhibitor
Selenomonas ruminantium
3.5.1.53
Mn2+
-
Selenomonas ruminantium
3.5.1.53
Mn2+
strong inhibitor
Selenomonas ruminantium
3.5.1.53
Zn2+
-
Selenomonas ruminantium
3.5.1.53
Zn2+
strong inhibitor
Selenomonas ruminantium
3.5.3.12
Al3+
1 mM
Selenomonas ruminantium
3.5.3.12
Co2+
1 mM
Selenomonas ruminantium
3.5.3.12
Cu2+
1 mM
Selenomonas ruminantium
3.5.3.12
Fe2+
1 mM
Selenomonas ruminantium
3.5.3.12
Fe3+
1 mM
Selenomonas ruminantium
3.5.3.12
Hg2+
strong inhibitor
Selenomonas ruminantium
3.5.3.12
iodoacetamide
strong inhibitor
Selenomonas ruminantium
3.5.3.12
Ni2+
1 mM
Selenomonas ruminantium
3.5.3.12
Zn2+
1 mM
Selenomonas ruminantium
4.1.1.19
DL-alpha-difluoromethylarginine
-
Selenomonas ruminantium
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.1.19
0.8
-
DL-alpha-difluoromethylarginine
-
Selenomonas ruminantium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.53
0.22
-
N-Carbamoylputrescine
-
Selenomonas ruminantium
3.5.3.12
6.67
-
agmatine
-
Selenomonas ruminantium
4.1.1.19
5.6
-
L-arginine
-
Selenomonas ruminantium
4.1.1.19
50
-
L-lysine
-
Selenomonas ruminantium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.53
32532
-
2 * 32532, calculated from sequence
Selenomonas ruminantium
3.5.1.53
37000
-
2 * 37000, SDS-PAGE
Selenomonas ruminantium
3.5.1.53
37000
-
SDS-PAGE
Selenomonas ruminantium
3.5.1.53
80000
-
gel filtration
Selenomonas ruminantium
3.5.3.12
45000
-
gel filtration; SDS-PAGE
Selenomonas ruminantium
4.1.1.19
58000
-
SDS-PAGE
Selenomonas ruminantium
4.1.1.19
120000
-
gel filtration
Selenomonas ruminantium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.53
-
Selenomonas ruminantium
3.5.1.53
recombinant
Selenomonas ruminantium
3.5.3.12
-
Selenomonas ruminantium
4.1.1.19
2024fold after final purification step with MonoQ HR 5/5
Selenomonas ruminantium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.19
0.00738
-
crude extract
Selenomonas ruminantium
4.1.1.19
0.01104
-
heat treatment
Selenomonas ruminantium
4.1.1.19
0.02394
-
after purification with DEAE-Toyopearl 650M
Selenomonas ruminantium
4.1.1.19
0.0381
-
after purification with DEAE-5PW
Selenomonas ruminantium
4.1.1.19
1.026
-
at the semi-final step of purification with HA-1000 column chromatography
Selenomonas ruminantium
4.1.1.19
14.94
-
final step of purification with MonoQ HR 5/5
Selenomonas ruminantium
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.19
4°C, 1 month, no lost of enzyme activity, pH 6.0-7.5
Selenomonas ruminantium
4.1.1.19
50°C, absence of PALP, 10 min, only 10% residual activity
Selenomonas ruminantium
4.1.1.19
70°C, presence of PALP, 60 min, no residual activity
Selenomonas ruminantium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.53
N-carbamoylputrescine + H2O
-
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
3.5.1.53
N-carbamoylputrescine + H2O
10 mM, assay at pH 6.5, 10 min, 40°C
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
25 mM, assay at pH 6.5, 10 min, 40°C
685687
Selenomonas ruminantium
putrescine + ?
-
-
-
?
4.1.1.19
L-arginine
assay at pH 6.5, 10 min, 50°C
685687
Selenomonas ruminantium
agmatine + CO2
-
-
-
?
4.1.1.19
L-lysine
assay at pH 6.5, 10 min, 50°C
685687
Selenomonas ruminantium
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.53
dimer
2 * 32532, calculated from sequence; 2 * 37000, SDS-PAGE
Selenomonas ruminantium
3.5.1.53
homodimer
-
Selenomonas ruminantium
3.5.3.12
monomer
-
Selenomonas ruminantium
4.1.1.19
dimer
-
Selenomonas ruminantium
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.53
45
-
-
Selenomonas ruminantium
3.5.1.53
45
-
assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
3.5.3.12
40
-
assay at
Selenomonas ruminantium
4.1.1.19
60
-
-
Selenomonas ruminantium
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.19
40
70
after 10 min incubation at 50°C 4fold higher enzyme activity than at 40°C, after 10 min incubation at 55°C 7fold higher enzyme activity than at 40°C, after 10 min incubation at 60°C 10fold higher enzyme activity than at 40°C, after 10 min incubation at 70°C 1.2fold higher enzyme activity than at 40°C
Selenomonas ruminantium
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.53
70
-
AguB activity is stable at up to 70°C for 30 min in 50 mM PIPES buffer, pH 6.5
Selenomonas ruminantium
3.5.1.53
70
-
up to 70°C, stable for 30 min at pH 6.5, 50 mM PIPES buffer
Selenomonas ruminantium
3.5.3.12
40
-
below 40°C stable for 30 min, pH 6.0-9.0
Selenomonas ruminantium
4.1.1.19
35
-
without pyridoxal 5'-phosphate, 50% redsidual enzyme activity
Selenomonas ruminantium
4.1.1.19
60
-
with pyridoxal 5'-phosphate, 54% residual enzyme activity
Selenomonas ruminantium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.53
7
-
-
Selenomonas ruminantium
3.5.1.53
7
-
assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
3.5.3.12
6.5
-
assay at
Selenomonas ruminantium
4.1.1.19
6.5
-
assay at pH 6.5, 10 min, 50°C
Selenomonas ruminantium
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
6.5
9
80% enzyme activity at pH 7.0, 70% enzyme activity at pH 8.0, 50% enzyme activity at pH 9.0
Selenomonas ruminantium
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.53
6
8
stable
Selenomonas ruminantium
3.5.1.53
6
8
-
Selenomonas ruminantium
3.5.3.12
6
9
30 min, temperature below 40°C
Selenomonas ruminantium
4.1.1.19
6.5
7.5
more than 80% enzyme activity, decreased enzyme activity at higher pH
Selenomonas ruminantium