BRENDA - Enzyme Database show

Chlorophenol hydroxylase activity encoded by TfdB from 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading Bradyrhizobium sp. strain RD5-C2

Huong, N.L.; Itoh, K.; Miyamoto, M.; Suyama, K.; Yamamoto, H.; Biosci. Biotechnol. Biochem. 71, 1691-1696 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.20
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue
Bradyrhizobium sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp.
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp. RD5-C2
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.20
Bradyrhizobium sp.
A5LGH2
gene tfdBa
-
1.14.13.20
Bradyrhizobium sp. RD5-C2
A5LGH2
gene tfdBa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
1.14.13.20
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp.
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp. RD5-C2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
1.14.13.20
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
1.14.13.20
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp.
?
-
-
-
-
1.14.13.20
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp. RD5-C2
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.20
25
-
assay at
Bradyrhizobium sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.20
7.6
-
assay at
Bradyrhizobium sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.20
NADPH
-
Bradyrhizobium sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.20
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue
Bradyrhizobium sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.20
NADPH
-
Bradyrhizobium sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp.
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp. RD5-C2
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
1.14.13.20
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp.
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp. RD5-C2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
1.14.13.20
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
1.14.13.20
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp.
?
-
-
-
-
1.14.13.20
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp. RD5-C2
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.20
25
-
assay at
Bradyrhizobium sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.20
7.6
-
assay at
Bradyrhizobium sp.