Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nishimoto, M.; Kitaoka, M.
    Identification of the putative proton donor residue of lacto-N-biose phosphorylase (EC 2.4.1.211) (2007), Biosci. Biotechnol. Biochem., 71, 1587-1591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.211 expression in Escherichia coli Bifidobacterium bifidum JCM 1254

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.211 D108N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D160N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D182N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D232N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D260N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D280N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D29N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D313N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D331N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D344N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D348N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D35N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D373N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D490N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D491N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 D561N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E249Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E259Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E319Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E356Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E377Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E475Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E568Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
2.4.1.211 E691Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.211 Bifidobacterium bifidum JCM 1254
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.211 of the recombinant proteins by Ni-NTA spin column chromatography Bifidobacterium bifidum JCM 1254

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.211 0.0002
-
D313N, no activity, D313 is probably the catalytic proton donor site Bifidobacterium bifidum JCM 1254
2.4.1.211 0.06
-
E356Q Bifidobacterium bifidum JCM 1254
2.4.1.211 0.17
-
D29N Bifidobacterium bifidum JCM 1254
2.4.1.211 0.22
-
D160N Bifidobacterium bifidum JCM 1254
2.4.1.211 0.31
-
E319Q Bifidobacterium bifidum JCM 1254
2.4.1.211 0.53
-
E568Q Bifidobacterium bifidum JCM 1254
2.4.1.211 0.72
-
D232N Bifidobacterium bifidum JCM 1254
2.4.1.211 0.9
-
D182N Bifidobacterium bifidum JCM 1254
2.4.1.211 1.68
-
E259Q Bifidobacterium bifidum JCM 1254
2.4.1.211 2.85
-
D280N Bifidobacterium bifidum JCM 1254
2.4.1.211 3.61
-
D35N Bifidobacterium bifidum JCM 1254
2.4.1.211 3.93
-
E377Q Bifidobacterium bifidum JCM 1254
2.4.1.211 4.79
-
E475Q Bifidobacterium bifidum JCM 1254
2.4.1.211 5.41
-
D491N Bifidobacterium bifidum JCM 1254
2.4.1.211 5.71
-
D348N Bifidobacterium bifidum JCM 1254
2.4.1.211 5.73
-
E249Q Bifidobacterium bifidum JCM 1254
2.4.1.211 5.84
-
E691Q Bifidobacterium bifidum JCM 1254
2.4.1.211 5.9
-
D561N Bifidobacterium bifidum JCM 1254
2.4.1.211 6.09
-
D331N Bifidobacterium bifidum JCM 1254
2.4.1.211 6.71
-
D490N Bifidobacterium bifidum JCM 1254
2.4.1.211 7.26
-
D108N Bifidobacterium bifidum JCM 1254
2.4.1.211 7.32
-
D344N Bifidobacterium bifidum JCM 1254
2.4.1.211 7.98
-
D373N Bifidobacterium bifidum JCM 1254
2.4.1.211 9.2
-
D260N Bifidobacterium bifidum JCM 1254
2.4.1.211 10
-
LNBP2 Bifidobacterium bifidum JCM 1254
2.4.1.211 10.5
-
LNBP1, all mutants display decreased activities, D313N has no activity Bifidobacterium bifidum JCM 1254

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
Bifidobacterium bifidum JCM 1254 alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Bifidobacterium bifidum JCM 1254 alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.211 lacto-N-biose phosphorylase
-
Bifidobacterium bifidum JCM 1254
2.4.1.211 lnba1 isoform LNBP1 Bifidobacterium bifidum JCM 1254
2.4.1.211 lnba2 isoform LNBP2 Bifidobacterium bifidum JCM 1254
2.4.1.211 LNBP
-
Bifidobacterium bifidum JCM 1254