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Literature summary extracted from

  • Ohnishi, T.; Watanabe, B.; Sakata, K.; Mizutani, M.
    CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato (2006), Biosci. Biotechnol. Biochem., 70, 2071-2080.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.178 expression in insect cell using a baculovirus expression system Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.178 microsome
-
 Solanum lycopersicum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.178 55000
-
 x * 55000, SDS-PAGE Solanum lycopersicum
1.14.14.178 56000
-
 x * 56000, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.178 additional information Solanum lycopersicum CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.178 Solanum lycopersicum A0ZS62 cv. Momotarou
-
1.14.14.178 Solanum lycopersicum A0ZS63 cv. Momotarou
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.178 flower
-
 Solanum lycopersicum
-
1.14.14.178 fruit transcript in mature fruit and in immature fruit Solanum lycopersicum
-
1.14.14.178 fruit transcript in mature fruit, no transcript detected in immature fruit Solanum lycopersicum
-
1.14.14.178 leaf young Solanum lycopersicum
-
1.14.14.178 seedling
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.178 (24R)-ergost-4-en-3-one + NADPH + H+ + O2 17% of the activity with campesterol Solanum lycopersicum (22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O
-
 ?
1.14.14.178 (24R)-ergost-4-en-3-one + NADPH + H+ + O2 7.9% of the activity with campesterol Solanum lycopersicum (22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O
-
 ?
1.14.14.178 campesterol + NADPH + H+ + O2 best substrate Solanum lycopersicum (22S)-22-hydroxycampesterol + NADP+ + H2O
-
 ?
1.14.14.178 additional information CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis Solanum lycopersicum ?
-
 ?
1.14.14.178 additional information the enzyme catalyzes C-22 hydroxylation of (14R)-5alpha-ergostan-3-one and campestanol at a trace level Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.178 ? x * 56000, SDS-PAGE Solanum lycopersicum
1.14.14.178 ? x * 55000, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
1.14.14.178 CYP724B2
-
 Solanum lycopersicum
1.14.14.178 CYP90B3
-
 Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.178 cytochrome P450
-
 Solanum lycopersicum
1.14.14.178 NADPH
-
 Solanum lycopersicum