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Literature summary extracted from

  • Liu, X.; Deng, G.; Chu, X.; Li, N.; Wu, L.; Li, D.
    Formation of an enolate intermediate is required for the reaction catalyzed by 3-hydroxyacyl-CoA dehydrogenase (2007), Bioorg. Med. Chem. Lett., 17, 3187-3190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.35 expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.35 N208A site-directed mutagenesis, the substrate binding of the mutant enzyme is affected Rattus norvegicus
1.1.1.35 S137A site-directed mutagenesis, the substrate binding of the mutant enzyme is affected Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 additional information
-
 additional information kinetics of wild-type and mutant enzymes, overview Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.35 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxyacyl-CoA + NAD+ Rattus norvegicus
-
 3-oxoacyl-CoA + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.35 (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+ reaction mechanism via enolate intermediate Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxyacyl-CoA + NAD+
-
 Rattus norvegicus 3-oxoacyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxyacyl-CoA + NAD+ the enzyme is a B-side-specific dehydrogenase with hydride transfer occurring on the si face of the nicotinamide ring Rattus norvegicus 3-oxoacyl-CoA + NADH + H+
-
 r
1.1.1.35 2-fluoro-3-hydroxy-4-octenoyl-CoA + NAD+
-
 Rattus norvegicus 2-fluoro-3-oxo-4-octenoyl-CoA + NADH
-
 r
1.1.1.35 2-fluoro-3-hydroxyoctanoyl-CoA + NAD+
-
 Rattus norvegicus 2-fluoro-3-oxooctanoyl-CoA + NADH
-
 r
1.1.1.35 3-hydroxy-4-octenoyl-CoA + NAD+
-
 Rattus norvegicus 3-oxo-4-octenoyl-CoA + NADH
-
 r
1.1.1.35 3-hydroxyoctanoyl-CoA + NAD+
-
 Rattus norvegicus 3-oxooctanoyl-CoA + NADH
-
 r
1.1.1.35 additional information no activity of the wild-type enzyme with 2,2-difluoro-3-hydroxyoctanoyl-CoA and 2,2-difluoro-3-hydroxy-4-octenoyl-CoA, substrate specificity of wild-type and mutant enzymes, y, overview Rattus norvegicus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Rattus norvegicus