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Literature summary extracted from

  • Ahn, M.; Cochrane, F.C.; Patchett, M.L.; Parker, E.J.
    Arabinose 5-phosphate analogues as mechanistic probes for Neisseria meningitidis 3-deoxy-D-manno-octulosonate 8-phosphate synthase (2008), Bioorg. Med. Chem., 16, 9830-9836.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 expressed in Escherichia coli BL21(DE3) cells Neisseria meningitidis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 N59A mutant retains less than 1% of the wild type activity Neisseria meningitidis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.001
-
 phosphoenolpyruvate Km less than 0.001 mM, in the presence of D-arabinose 5-phosphate, at 30°C Neisseria meningitidis
2.5.1.55 0.0025
-
 phosphoenolpyruvate in the presence of L-xylose 5-phosphate, at 30°C Neisseria meningitidis
2.5.1.55 0.012
-
 D-arabinose 5-phosphate at 30°C Neisseria meningitidis
2.5.1.55 0.057
-
 L-xylose 5-phosphate at 30°C Neisseria meningitidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 30000
-
 SDS-PAGE Neisseria meningitidis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Neisseria meningitidis Q9JZ55
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 Source 15Q column chromatography and Source 15Phe column chromatography Neisseria meningitidis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.55 0.6
-
 crude cell lysate, at 25°C and pH 7.5 Neisseria meningitidis
2.5.1.55 2
-
 purified enzyme, at 25°C and pH 7.5 Neisseria meningitidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 additional information D-ribose 5-phosphate, D-lyxose 5-phosphate, and D-xylose 5-phosphate with altered configuration at C2, C3 or both C2 and C3 relative to D-arabinose 5-phosphate are no substrates for KDO8P synthase Neisseria meningitidis ?
-
 ?
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Neisseria meningitidis 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?
2.5.1.55 phosphoenolpyruvate + L-xylose 5-phosphate + H2O
-
 Neisseria meningitidis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 3-deoxy-D-manno-octulosonate 8-phosphate synthase
-
 Neisseria meningitidis
2.5.1.55 Kdo8P synthase
-
 Neisseria meningitidis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 1.1
-
 L-xylose 5-phosphate at 30°C Neisseria meningitidis
2.5.1.55 2.7
-
 D-arabinose 5-phosphate at 30°C Neisseria meningitidis