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Literature summary extracted from

  • Brunskole, M.; Stefane, B.; Zorko, K.; Anderluh, M.; Stojan, J.; Lanisnik Rizner, T.; Gobec, S.
    Towards the first inhibitors of trihydroxynaphthalene reductase from Curvularia lunata: Synthesis of artificial substrate, homology modelling and initial screening (2008), Bioorg. Med. Chem., 16, 5881-5889.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.252 drug development the enzyme represents a target for the development of fungicides and antimicotics Curvularia lunata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.252 GST-tagged enzyme expression in Escherichia coli Curvularia lunata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.252 1H-indene-1,3(2H)-dione
-
 Curvularia lunata
1.1.1.252 2,3-Dihydro-2,5-dihydroxy-4H-benzopyran-4-one DDBO, an artificial substrate, chemical synthesis method development, overview Curvularia lunata
1.1.1.252 diclofenac
-
 Curvularia lunata
1.1.1.252 ethyl 6-methyl-4-oxo-4H-chromene-2-carboxylate
-
 Curvularia lunata
1.1.1.252 ethyl 6-methyl-8-nitro-4-oxo-4H-chromene-2-carboxylate
-
 Curvularia lunata
1.1.1.252 etodolac
-
 Curvularia lunata
1.1.1.252 nabumetone
-
 Curvularia lunata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.252 1,3,8-trihydroxynaphthalene + NADPH + H+ Curvularia lunata the enzyme is an essential enzyme in the biosynthesis of fungal melanin vermelone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.252 Curvularia lunata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.252 recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography Curvularia lunata

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.252 scytalone + NADP+ = 1,3,6,8-tetrahydroxynaphthalene + NADPH + H+ the catalytic triad is formed by residues S149, Y163, and K167 Curvularia lunata
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.252 1,3,8-trihydroxynaphthalene + NADPH + H+
-
 Curvularia lunata vermelone + NADP+
-
 ?
1.1.1.252 1,3,8-trihydroxynaphthalene + NADPH + H+ the enzyme is an essential enzyme in the biosynthesis of fungal melanin Curvularia lunata vermelone + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.252 More enzyme homology modelling, overview Curvularia lunata

Synonyms

EC Number Synonyms Comment Organism
1.1.1.252 3HNR
-
 Curvularia lunata
1.1.1.252 trihydroxynaphthalene reductase
-
 Curvularia lunata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.252 7
-
 assay at Curvularia lunata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.252 NADPH
-
 Curvularia lunata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.252 0.017
-
 1H-indene-1,3(2H)-dione pH 7.0 Curvularia lunata
1.1.1.252 0.017
-
 etodolac pH 7.0 Curvularia lunata
1.1.1.252 0.108
-
 diclofenac pH 7.0 Curvularia lunata
1.1.1.252 0.108
-
 ethyl 6-methyl-4-oxo-4H-chromene-2-carboxylate pH 7.0 Curvularia lunata
1.1.1.252 0.111
-
 nabumetone pH 7.0 Curvularia lunata
1.1.1.252 0.111
-
 ethyl 6-methyl-8-nitro-4-oxo-4H-chromene-2-carboxylate pH 7.0 Curvularia lunata