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Literature summary extracted from

  • Matsumoto, K.; Takase, K.; Aoki, E.; Doi, Y.; Taguchi, S.
    Synergistic effects of Glu130Asp substitution in the type II polyhydroxyalkanoate (PHA) synthase: enhancement of PHA production and alteration of polymer molecular weight (2005), Biomacromolecules, 6, 99-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 recombinant Escherichia coli strain JM109 harboring the E130D mutant gene accumulates 10fold higher (1.0 wt%) poly(3-hydroxybutyrate) from glucose, compared to recombinant Escherichia coli harboring the wild-type PHA synthase gene (0.1 wt%). Recombinant Escherichia coli strain LS5218 harboring the E130D PHA synthase gene grown on dodecanoate produces more poly(3-hydroxybutanoate-co-3-hydroxyalkanoate) (20 wt%) copolymer than an LS5218 strain harboring the wild-type PHA synthase gene (13 wt%). The E130D mutation also results in the production of copolymer with a slight increase in 3-hydroxybutanoate composition, compared to copolymer produced by the wild-type PHA synthase Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 E130D recombinant Escherichia coli strain JM109 harboring the E130D mutant gene accumulates 10fold higher (1.0 wt%) poly(3-hydroxybutyrate) from glucose, compared to recombinant Escherichia coli harboring the wild-type PHA synthase gene (0.1 wt%). Recombinant Escherichia coli strain LS5218 harboring the E130D PHA synthase gene grown on dodecanoate produces more poly(3-hydroxybutanoate-co-3-hydroxyalkanoate) (20 wt%) copolymer than an LS5218 strain harboring the wild-type PHA synthase gene (13 wt%). The E130D mutation also results in the production of copolymer with a slight increase in 3-hydroxybutanoate composition, compared to copolymer produced by the wild-type PHA synthase. Mutation results in the production of copolymer with a slight increase in 3-hydroxybutanoate composition, compared to copolymer produced by the wild-type PHA synthase. In vitro enzyme activities of the E130D PHA synthase toward various 3-hydroxyacyl-CoAs (4-10 carbons in length) are all higher than those of the wild-type enzyme. Mutation decreases the molecular weight of poly(3-hydroxybutyrate) Pseudomonas sp.
2.3.1.304 E130D/Q481K E130D mutation decreases the molecular weight of poly(3-hydroxybutyrate). If the mutation is combined with the Q481K mutation, the enzyme can produce poly(3-hydroxybutyrate) with higher molecular weight Pseudomonas sp.
2.3.1.304 E130D/Q481M the double mutant shows much higher poly(3-hydroxybutyrate) accumulation (29-34 wt%) compared to poly(3-hydroxybutyrate) accumulation in cells harboring PHA synthase with the individual mutations E130D or Q481M alone Pseudomonas sp.
2.3.1.304 E130D/Q481R the double mutant shows much higher poly(3-hydroxybutyrate) accumulation (29-34 wt%) compared to poly(3-hydroxybutyrate) accumulation in cells harboring PHA synthase with the individual mutations E130D or Q481R alone Pseudomonas sp.
2.3.1.304 E130D/S325C the double mutants of E130D with either the S325T or the S325C mutations exhibits strong synergistic increases in poly(3-hydroxybutyrate) content, up to 39 wt% Pseudomonas sp.
2.3.1.304 E130D/S325T the double mutants of E130D with either the S325T or the S325C mutations exhibits strong synergistic increases in poly(3-hydroxybutyrate) content, up to 39 wt% Pseudomonas sp.
2.3.1.304 Q481K mutation decreases the molecular weight of poly(3-hydroxybutyrate) Pseudomonas sp.
2.3.1.304 S325T mutation decreases the molecular weight of poly(3-hydroxybutyrate) Pseudomonas sp.
2.3.1.304 S325T/Q481K S325T mutation decreases the molecular weight of poly(3-hydroxybutyrate). If the mutation is combined with the Q481K mutation, the enzyme can produce poly(3-hydroxybutyrate) with higher molecular weight Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Pseudomonas sp.
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
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Pseudomonas sp. [(R)-3-hydroxybutanoate](n+1) + CoA
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Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 type II PHA synthase
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Pseudomonas sp.
2.3.1.304 type II polyhydroxyalkanoate synthase
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Pseudomonas sp.