EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.304 | recombinant Escherichia coli strain JM109 harboring the E130D mutant gene accumulates 10fold higher (1.0 wt%) poly(3-hydroxybutyrate) from glucose, compared to recombinant Escherichia coli harboring the wild-type PHA synthase gene (0.1 wt%). Recombinant Escherichia coli strain LS5218 harboring the E130D PHA synthase gene grown on dodecanoate produces more poly(3-hydroxybutanoate-co-3-hydroxyalkanoate) (20 wt%) copolymer than an LS5218 strain harboring the wild-type PHA synthase gene (13 wt%). The E130D mutation also results in the production of copolymer with a slight increase in 3-hydroxybutanoate composition, compared to copolymer produced by the wild-type PHA synthase | Pseudomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.304 | E130D | recombinant Escherichia coli strain JM109 harboring the E130D mutant gene accumulates 10fold higher (1.0 wt%) poly(3-hydroxybutyrate) from glucose, compared to recombinant Escherichia coli harboring the wild-type PHA synthase gene (0.1 wt%). Recombinant Escherichia coli strain LS5218 harboring the E130D PHA synthase gene grown on dodecanoate produces more poly(3-hydroxybutanoate-co-3-hydroxyalkanoate) (20 wt%) copolymer than an LS5218 strain harboring the wild-type PHA synthase gene (13 wt%). The E130D mutation also results in the production of copolymer with a slight increase in 3-hydroxybutanoate composition, compared to copolymer produced by the wild-type PHA synthase. Mutation results in the production of copolymer with a slight increase in 3-hydroxybutanoate composition, compared to copolymer produced by the wild-type PHA synthase. In vitro enzyme activities of the E130D PHA synthase toward various 3-hydroxyacyl-CoAs (4-10 carbons in length) are all higher than those of the wild-type enzyme. Mutation decreases the molecular weight of poly(3-hydroxybutyrate) | Pseudomonas sp. |
2.3.1.304 | E130D/Q481K | E130D mutation decreases the molecular weight of poly(3-hydroxybutyrate). If the mutation is combined with the Q481K mutation, the enzyme can produce poly(3-hydroxybutyrate) with higher molecular weight | Pseudomonas sp. |
2.3.1.304 | E130D/Q481M | the double mutant shows much higher poly(3-hydroxybutyrate) accumulation (29-34 wt%) compared to poly(3-hydroxybutyrate) accumulation in cells harboring PHA synthase with the individual mutations E130D or Q481M alone | Pseudomonas sp. |
2.3.1.304 | E130D/Q481R | the double mutant shows much higher poly(3-hydroxybutyrate) accumulation (29-34 wt%) compared to poly(3-hydroxybutyrate) accumulation in cells harboring PHA synthase with the individual mutations E130D or Q481R alone | Pseudomonas sp. |
2.3.1.304 | E130D/S325C | the double mutants of E130D with either the S325T or the S325C mutations exhibits strong synergistic increases in poly(3-hydroxybutyrate) content, up to 39 wt% | Pseudomonas sp. |
2.3.1.304 | E130D/S325T | the double mutants of E130D with either the S325T or the S325C mutations exhibits strong synergistic increases in poly(3-hydroxybutyrate) content, up to 39 wt% | Pseudomonas sp. |
2.3.1.304 | Q481K | mutation decreases the molecular weight of poly(3-hydroxybutyrate) | Pseudomonas sp. |
2.3.1.304 | S325T | mutation decreases the molecular weight of poly(3-hydroxybutyrate) | Pseudomonas sp. |
2.3.1.304 | S325T/Q481K | S325T mutation decreases the molecular weight of poly(3-hydroxybutyrate). If the mutation is combined with the Q481K mutation, the enzyme can produce poly(3-hydroxybutyrate) with higher molecular weight | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.304 | Pseudomonas sp. | - |
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- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.304 | (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Pseudomonas sp. | [(R)-3-hydroxybutanoate](n+1) + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.304 | type II PHA synthase | - |
Pseudomonas sp. |
2.3.1.304 | type II polyhydroxyalkanoate synthase | - |
Pseudomonas sp. |