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Literature summary extracted from

  • Song, J.J.; Zhang, S.; Lenz, R.W.; Goodwin, S.
    In vitro polymerization and copolymerization of 3-hydroxypropionyl-CoA with the PHB synthase from Ralstonia eutropha (2000), Biomacromolecules, 1, 433-439.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 expressed in Escherichia coli Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.304 CoA very effective competitive inhibitor for the polymerization of 3-hydroxypropionyl-CoA Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.304 0.189
-
 3-hydroxypropionyl-CoA
-
 Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.304 recombinant Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 3-hydroxypropanoyl-CoA + (3-hydroxypropanoate)n the value of the number-average molecular weight of the polymer obtained at a molar ratio of monomer-to-enzyme of 5000 is 25000 while the polydispersity is 4.7 Cupriavidus necator [(R)-3-hydroxypropanoate](n+1) + CoA
-
 ?
2.3.1.304 3-hydroxypropanoyl-CoA + 3-hydroxybutanoyl-CoA
-
 Cupriavidus necator poly(3-hydroxypropanoate-co-3-hydroxybutanoate) + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 PHB synthase
-
 Cupriavidus necator
2.3.1.304 poly(3-hydroxybutyrate) synthase
-
 Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.304 10
-
 3-hydroxypropionyl-CoA
-
 Cupriavidus necator

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.304 0.085
-
 CoA
-
 Cupriavidus necator