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Literature summary extracted from

  • Bubinas, A.; Giedraityte, G.; Kalediene, L.
    Protocatechuate 3,4-dioxygenase from thermophilic Geobacillus sp. strain (2007), Biologija (Vilnius), 53, 31-34.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.13.11.3 environmental protection the purified enzyme can be used in bioremediation of polluted groundwater or soil contaminated with various aromatic compounds ranging from monocyclic to polycyclic Geobacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.007
-
 3,4-dihydroxybenzoate
-
 Geobacillus sp.
1.13.11.3 0.033
-
 catechol
-
 Geobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.3 480000
-
 gel filtration Geobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Geobacillus sp.
-
 strain AY946034
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3
-
 Geobacillus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.3 culture-condition:naphthalene-grown cell
-
 Geobacillus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 34.2
-
-
 Geobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Geobacillus sp. 3-carboxy-cis,cis-muconate
-
 ?
1.13.11.3 catechol + O2
-
 Geobacillus sp. muconate
-
 ?
1.13.11.3 additional information no activity with gentisate Geobacillus sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.3 60
-
-
 Geobacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.3 50 70 50°C: about 55% of maximal activity, 70°C: about 60% of maximal activity Geobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.3 60
-
 half-life is 40 min Geobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 8
-
-
 Geobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.3 6 9 pH 6.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity Geobacillus sp.