Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bylander, J.E.; Bertenshaw, G.P.; Matters, G.L.; Hubbard, S.J.; Bond, J.S.
    Human and mouse homo-oligomeric meprin A metalloendopeptidase: substrate and inhibitor specificities (2007), Biol. Chem., 388, 1163-1172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.18 actinonin
-
 Homo sapiens
3.4.24.18 actinonin
-
 Mus musculus
3.4.24.18 captopril
-
 Homo sapiens
3.4.24.18 CGS 27023A
-
 Homo sapiens
3.4.24.18 CGS 27023A
-
 Mus musculus
3.4.24.18 CONA 65
-
 Homo sapiens
3.4.24.18 CONA 65
-
 Mus musculus
3.4.24.18 CONA 68
-
 Homo sapiens
3.4.24.18 CONA 68
-
 Mus musculus
3.4.24.18 CONA 71
-
 Homo sapiens
3.4.24.18 CONA 71
-
 Mus musculus
3.4.24.18 CONA 73
-
 Homo sapiens
3.4.24.18 CONA 73
-
 Mus musculus
3.4.24.18 doxycycline
-
 Homo sapiens
3.4.24.18 doxycycline
-
 Mus musculus
3.4.24.18 GM6001 Ilomastat Homo sapiens
3.4.24.18 GM6001 Ilomastat Mus musculus
3.4.24.18 homophenylalanine hydroxamate
-
 Homo sapiens
3.4.24.18 homophenylalanine hydroxamate
-
 Mus musculus
3.4.24.18 Pro-Leu-Gly hydroxamate
-
 Homo sapiens
3.4.24.18 Pro-Leu-Gly hydroxamate
-
 Mus musculus
3.4.24.18 TNF-a processing enzyme inhibitor TAPI-2 Homo sapiens
3.4.24.18 TNF-alpha processing enzyme inhibitor TAPI-2 Mus musculus
3.4.24.18 tyrosine hydroxamate
-
 Homo sapiens
3.4.24.18 tyrosine hydroxamate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.18 0.018
-
 protein PTH12-34 hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.0223
-
 alpha-MSH meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Mus musculus
3.4.24.18 0.0296
-
 protein GRP meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Mus musculus
3.4.24.18 0.0306
-
 Substance P meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Mus musculus
3.4.24.18 0.0339
-
 secretin hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.0413
-
 Substance P hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.0565
-
 luteinizing hormone releasing hormone LHRH hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.0672
-
 protein PTH12-34 meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Mus musculus
3.4.24.18 0.0732
-
 orcokinin hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.101
-
 bradykinin meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Mus musculus
3.4.24.18 0.11
-
 GRP hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.111
-
 secretin meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Mus musculus
3.4.24.18 0.125
-
 bradykinin hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.156
-
 luteinizing hormone releasing hormone LHRH meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 for between 8-30 min Mus musculus
3.4.24.18 0.2
-
 gastrin hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens
3.4.24.18 0.292
-
 alpha-MSH hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.18 86000
-
 monomeric molecular mass of a meprin alpha-subunit Mus musculus
3.4.24.18 86000
-
 monomeric molecular mass of a meprin alpha-subunit Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.18 Homo sapiens
-
-
-
3.4.24.18 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.18 HEK-293 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.18 2.9
-
 specific activity of homo-oligomeric meprin A against PTH Homo sapiens
3.4.24.18 4.2
-
 specific activity of homo-oligomeric meprin A against PTH Mus musculus
3.4.24.18 2700
-
 specific activity of homo-oligomeric meprin A against gelatin Homo sapiens
3.4.24.18 20500
-
 specific activity of homo-oligomeric meprin A against azocasein Mus musculus
3.4.24.18 20800
-
 specific activity of homo-oligomeric meprin A against azocasein Homo sapiens
3.4.24.18 25000
-
 specific activity of homo-oligomeric meprin A against gelatin Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.18 alpha-MSH + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 alpha-MSH + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 bradykinin + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 bradykinin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 gastrin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 GRP + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 luteinizing hormone releasing hormone LHRH + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 luteinizing hormone releasing hormone LHRH + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 orcokinin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 protein GRP + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 protein GRP + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 protein LHRH + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 protein LHRH + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 protein PTH12-34 + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 protein PTH12-34 + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 Secretin + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 Secretin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.18 Substance P + H2O
-
 Mus musculus ?
-
 ?
3.4.24.18 Substance P + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.18 0.4
-
 gastrin
-
 Homo sapiens
3.4.24.18 3.7
-
 bradykinin
-
 Homo sapiens
3.4.24.18 4.4
-
 secretin
-
 Mus musculus
3.4.24.18 4.6
-
 parathyphoid hormone 12-34
-
 Homo sapiens
3.4.24.18 5.1
-
 protein LHRH
-
 Homo sapiens
3.4.24.18 5.2
-
 Substance P
-
 Homo sapiens
3.4.24.18 6.3
-
 orcokinin
-
 Homo sapiens
3.4.24.18 8.4
-
 secretin
-
 Homo sapiens
3.4.24.18 10.5
-
 protein PTH12-34
-
 Mus musculus
3.4.24.18 12.1
-
 Substance P
-
 Mus musculus
3.4.24.18 16.5
-
 alpha-MSH
-
 Homo sapiens
3.4.24.18 16.6
-
 bradykinin
-
 Mus musculus
3.4.24.18 19.6
-
 protein GRP
-
 Homo sapiens
3.4.24.18 24.6
-
 protein GRP
-
 Mus musculus
3.4.24.18 39.4
-
 protein LHRH
-
 Mus musculus
3.4.24.18 45.4
-
 alpha-MSH
-
 Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.18 0.0033
-
-
 Mus musculus CONA 65
3.4.24.18 0.005
-
-
 Homo sapiens actinonin
3.4.24.18 0.008
-
-
 Mus musculus CGS 27023A
3.4.24.18 0.016
-
-
 Homo sapiens CGS 27023A
3.4.24.18 0.04
-
-
 Mus musculus CONA 68
3.4.24.18 0.05
-
-
 Homo sapiens CONA 68
3.4.24.18 0.06
-
 Ilomastat Homo sapiens GM6001
3.4.24.18 0.065
-
-
 Homo sapiens CONA 73
3.4.24.18 0.08
-
-
 Homo sapiens TNF-alpha processing enzyme inhibitor
3.4.24.18 0.1
-
-
 Homo sapiens tyrosine hydroxamate
3.4.24.18 0.1
-
-
 Homo sapiens homophenylalanine hydroxamate
3.4.24.18 0.17
-
-
 Homo sapiens CONA 71
3.4.24.18 0.18
-
-
 Mus musculus actinonin
3.4.24.18 0.25
-
-
 Mus musculus CONA 71
3.4.24.18 0.31
-
-
 Mus musculus CONA 73
3.4.24.18 0.4
-
-
 Homo sapiens Pro-Leu-Gly hydroxamate
3.4.24.18 0.6
-
 Ilomastat Mus musculus GM6001
3.4.24.18 0.8
-
-
 Mus musculus homophenylalanine hydroxamate
3.4.24.18 0.8
-
-
 Mus musculus TNF-alpha processing enzyme inhibitor
3.4.24.18 1.1
-
-
 Mus musculus tyrosine hydroxamate
3.4.24.18 2
-
-
 Homo sapiens CONA 65
3.4.24.18 12
-
-
 Homo sapiens doxycycline
3.4.24.18 16
-
-
 Mus musculus Pro-Leu-Gly hydroxamate
3.4.24.18 20
-
-
 Mus musculus doxycycline
3.4.24.18 40
-
-
 Homo sapiens captopril