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Literature summary extracted from

  • Viggor, S.; Heinaru, E.; Kuennapas, A.; Heinaru, A.
    Evaluation of different phenol hydroxylase-possessing phenol-degrading pseudomonads by kinetic parameters (2008), Biodegradation, 19, 759-769.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.7 degradation phenol degradation, among kinetic parameters of growth, the maximum specific growth rate significantly affects the rate of contaminant degradation and is therefore an important parameter to characterise microbes in biological reatment systems Pseudomonas fluorescens
1.14.13.7 degradation phenol degradation, among kinetic parameters of growth, the maximum specific growth rate significantly affects the rate of contaminant degradation and is therefore an important parameter to characterise microbes in biological reatment systems Pseudomonas putida
1.14.13.7 degradation phenol degradation, among kinetic parameters of growth, the maximum specific growth rate significantly affects the rate of contaminant degradation and is therefore an important parameter to characterise microbes in biological reatment systems Pseudomonas mendocina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 DNA and amino acid sequence determination and analysis, expression analysis Pseudomonas mendocina
1.14.13.7 gene pheBA, DNA and amino acid sequence determination and analysis, expression analysis Pseudomonas fluorescens
1.14.13.7 gene pheBA, DNA and amino acid sequence determination and analysis, expression analysis Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.7 phenol
-
 Pseudomonas fluorescens
1.14.13.7 phenol
-
 Pseudomonas mendocina
1.14.13.7 phenol
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.7 additional information
-
 additional information kinetic analysis Pseudomonas mendocina
1.14.13.7 additional information
-
 additional information kinetic analysis of the enzyme from different strains, overview Pseudomonas fluorescens
1.14.13.7 additional information
-
 additional information kinetic analysis of the enzyme from different strains, overview Pseudomonas putida
1.14.13.7 0.0013
-
 phenol strain B PC30 Pseudomonas putida
1.14.13.7 0.0014
-
 phenol strain B PC18 Pseudomonas fluorescens
1.14.13.7 0.0017
-
 phenol strain PC1 Pseudomonas mendocina
1.14.13.7 0.0019
-
 phenol strain F PC17 Pseudomonas fluorescens
1.14.13.7 0.0024
-
 phenol strain B PC16 Pseudomonas putida
1.14.13.7 0.0128
-
 phenol strain C PC31 Pseudomonas fluorescens
1.14.13.7 0.0128
-
 phenol strain EST1412 Pseudomonas putida
1.14.13.7 0.0131
-
 phenol strain F PC20 Pseudomonas fluorescens
1.14.13.7 0.0214
-
 phenol strain C PC24 Pseudomonas fluorescens
1.14.13.7 0.0271
-
 phenol strain F P69 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 phenol + NADH + H+ + O2 Pseudomonas fluorescens
-
 catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2 Pseudomonas putida
-
 catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2 Pseudomonas mendocina
-
 catechol + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Pseudomonas fluorescens
-
 gene pheBA, single and multicomponent enzyme containing strains C PC24, F PC20, C PC31, F P69, B PC18, and F PC17
-
1.14.13.7 Pseudomonas mendocina
-
 multicomponent enzyme containing strain PC1
-
1.14.13.7 Pseudomonas putida
-
 gene pheBA, single and multicomponent enzyme containing strains B PC30, B PC16, and EST1412
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 phenol + NADH + H+ + O2
-
 Pseudomonas fluorescens catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2
-
 Pseudomonas putida catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2
-
 Pseudomonas mendocina catechol + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 Mph
-
 Pseudomonas fluorescens
1.14.13.7 Mph
-
 Pseudomonas putida
1.14.13.7 Mph
-
 Pseudomonas mendocina
1.14.13.7 multicomponent PH
-
 Pseudomonas fluorescens
1.14.13.7 multicomponent PH
-
 Pseudomonas putida
1.14.13.7 multicomponent PH
-
 Pseudomonas mendocina
1.14.13.7 phenol hydroxylase
-
 Pseudomonas fluorescens
1.14.13.7 phenol hydroxylase
-
 Pseudomonas putida
1.14.13.7 phenol hydroxylase
-
 Pseudomonas mendocina
1.14.13.7 single-component PH
-
 Pseudomonas fluorescens
1.14.13.7 single-component PH
-
 Pseudomonas putida
1.14.13.7 SPH
-
 Pseudomonas fluorescens
1.14.13.7 SPH
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADH
-
 Pseudomonas fluorescens
1.14.13.7 NADH
-
 Pseudomonas putida
1.14.13.7 NADH
-
 Pseudomonas mendocina

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.7 1.99
-
 phenol strain F P69 Pseudomonas fluorescens
1.14.13.7 2.52
-
 phenol strain B PC18 Pseudomonas fluorescens
1.14.13.7 3.44
-
 phenol strain F PC20 Pseudomonas fluorescens
1.14.13.7 3.56
-
 phenol strain F PC17 Pseudomonas fluorescens
1.14.13.7 3.84
-
 phenol strain B PC16 Pseudomonas putida
1.14.13.7 4.31
-
 phenol strain C PC31 Pseudomonas fluorescens
1.14.13.7 4.4
-
 phenol strain B PC30 Pseudomonas putida
1.14.13.7 7.44
-
 phenol strain C PC24 Pseudomonas fluorescens
1.14.13.7 7.57
-
 phenol strain EST1412 Pseudomonas putida
1.14.13.7 9.41
-
 phenol strain PC1 Pseudomonas mendocina