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Literature summary extracted from

  • Ohlsson, L.; Palmberg, C.; Duan, R.D.; Olsson, M.; Bergman, T.; Nilsson, A.
    Purification and characterization of human intestinal neutral ceramidase (2007), Biochimie, 89, 950-960.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 CHAPS 20 mM, 50% inhibition Homo sapiens
3.5.1.23 cholesterol 0.08 mM or below, decreases the hydrolysis of ceramide by a maximum of two-thirds Homo sapiens
3.5.1.23 Cu2+
-
 Homo sapiens
3.5.1.23 glycodeoxycholate
-
 Homo sapiens
3.5.1.23 Mg2+ 5 mM Homo sapiens
3.5.1.23 Mn2+ 5 mM Homo sapiens
3.5.1.23 taurocholate 20 mM, 50% inhibition Homo sapiens
3.5.1.23 taurodeoxycholate
-
 Homo sapiens
3.5.1.23 Zn2+
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.01302
-
 N-octanoylsphingosine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 116000
-
 x * 116000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 additional information Homo sapiens neutral ceramidase plays a major role in ceramide metabolism in the human gut ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein deglycosylation does not affect the activity Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 ileostomal fluid
-
 Homo sapiens
-
3.5.1.23 intestine neutral ceramidase is expressed in the intestine and released in the intestinal lumen Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 3.647
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 additional information neutral ceramidase plays a major role in ceramide metabolism in the human gut Homo sapiens ?
-
 ?
3.5.1.23 N-octanoylsphingosine + H2O
-
 Homo sapiens octanoic acid + sphingosine
-
 r
3.5.1.23 N-oleoylsphingosine + H2O
-
 Homo sapiens oleic acid + sphingosine
-
 r
3.5.1.23 N-palmitoylsphingosine + H2O
-
 Homo sapiens palmitic acid + sphingosine
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 116000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 neutral ceramidase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 6.8
-
 synthesis of N-palmitoylsphingosine Homo sapiens
3.5.1.23 7.5
-
 hydrolysis of N-octanoylsphingosine Homo sapiens