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Literature summary extracted from

  • Kumar, A.K.; Goswami, P.
    Purification and properties of a novel broad substrate specific alcohol oxidase from Aspergillus terreus MTCC 6324 (2008), Biochim. Biophys. Acta, 1784, 1552-1559.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.13 Ferrocene 1 mM, increases the alcohol oxidase activity only for the short chain alcohol substrate to 47% of the original activity Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 1,12-dichlorododecane
-
 Aspergillus terreus
1.1.3.13 2-mercaptoethanol
-
 Aspergillus terreus
1.1.3.13 AgNO3 1 mM, complete loss of activity Aspergillus terreus
1.1.3.13 PCMB 5 mM, complete loss of activity Aspergillus terreus
1.1.3.13 Tetraethylthiuram disulfide 5 mM, more than 70% loss of activity Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.498
-
 n-Heptanol
-
 Aspergillus terreus
1.1.3.13 1.86
-
 n-dodecanol
-
 Aspergillus terreus
1.1.3.13 2.3
-
 phenyl-3-propanol
-
 Aspergillus terreus
1.1.3.13 18.3
-
 (R)-2-octanol
-
 Aspergillus terreus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.13 membrane
-
 Aspergillus terreus 16020
-
1.1.3.13 microsome
-
 Aspergillus terreus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.13 MgCl2 1 mM, increases the enzyme activity to nearly 50% for both short chain-, and long chain alcohol substrates Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 13000
-
 x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE Aspergillus terreus
1.1.3.13 27000
-
 x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE Aspergillus terreus
1.1.3.13 43000
-
 x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE Aspergillus terreus
1.1.3.13 63000
-
 x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE Aspergillus terreus
1.1.3.13 85000
-
 x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE Aspergillus terreus
1.1.3.13 269000
-
 non-denaturing PAGE Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Aspergillus terreus
-
 MTCC 6324
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.13 lipoprotein presence of oleic acid and palmitic acid at a ratio of 2:1 in the purified protein. This lipoidic nature of the protein particles is correlated to the high aggregating property Aspergillus terreus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Aspergillus terreus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 culture condition:n-hexadecane grown-cell
-
 Aspergillus terreus
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.13 4°C, 1 month, more than 50% of the activity is retained Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 (R)-2-octanol + O2
-
 Aspergillus terreus 2-octanone + H2O2
-
 ?
1.1.3.13 n-dodecanol + O2
-
 Aspergillus terreus n-dodecanal + H2O2
-
 ?
1.1.3.13 n-heptanol + O2 substrates with highest affinity Aspergillus terreus n-heptanal + H2O2
-
 ?
1.1.3.13 phenyl-3-propanol + O2
-
 Aspergillus terreus 3-phenylpropanal
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 ? x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 broad substrate specific alcohol oxidase
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 4
-
 1 month, more than 50% of the activity is retained Aspergillus terreus
1.1.3.13 32
-
 2 h, 80% loss of activity Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.13 270
-
 n-Heptanol
-
 Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 9
-
 3 h, 5% loss of activity Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD flavin is non-covalently but avidly associated. Peptide mass fingerprinting studies show the presence of two FAD binding domains in 63 kDa protein Aspergillus terreus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.13 0.000014
-
 2-mercaptoethanol substrate: R-(-)-2-octanol Aspergillus terreus
1.1.3.13 0.00063
-
 2-mercaptoethanol substrate: n-dodecanol Aspergillus terreus
1.1.3.13 0.00157
-
 2-mercaptoethanol substrate: n-heptanol Aspergillus terreus
1.1.3.13 0.00246
-
 1,12-dichlorododecane substrate: n-dodecanal Aspergillus terreus
1.1.3.13 0.00362
-
 2-mercaptoethanol substrate: phenyl-3-propanol Aspergillus terreus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.13 Aspergillus terreus isoelectric focusing, pH-range: 3-10 8.5 8.3