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Literature summary extracted from

  • Crawley, S.W.; Cote, G.P.
    Determinants for substrate phosphorylation by Dictyostelium myosin II heavy chain kinases A and B and eukaryotic elongation factor-2 kinase (2008), Biochim. Biophys. Acta, 1784, 908-915.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 Calmodulin
-
 Dictyostelium discoideum
2.7.11.20 Calmodulin required Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.7 expression of MHCK A, comprising residues 527-887, i.e. A-CAT, as a GST-fusion protein, phylogenetic analysis Dictyostelium discoideum
2.7.11.7 expression of MHCK B, comprising residues 13-459, i.e. B-CAT, as a GST-fusion protein, phylogenetic analysis Dictyostelium discoideum
2.7.11.20 enzyme expression as GST-tagged protein in Escherichia coli strain BL21(DE3) Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 additional information
-
 additional information kinetics Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+
-
 Dictyostelium discoideum
2.7.11.20 Ca2+ required Mus musculus
2.7.11.20 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin heavy-chain Dictyostelium discoideum
-
 ADP + myosin heavy chain phosphate
-
 ?
2.7.11.20 ATP + [eukaryotic translation elongation factor 2] Mus musculus eEF2 is involved in eEF2 in neurite outgrowth regulation mechanism, since phosphorylated eEF2 inhibits mRNA translation via inhibition of eEF2-ribosome binding, calcium elevation appears to inhibit mRNA translation in growth cones by a synergistic mechanism involving regulation of EF2K, S6K, and eEF2 itself, overview ADP + [eukaryotic translation elongation factor 2] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum P42527 MHCK A
-
2.7.11.7 Dictyostelium discoideum P90648 MHCK B
-
2.7.11.20 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein phosphorylation by p70-S6 kinase inhibits EF2K, Ca2+ decreases the phosphorylation of the enzyme Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 recombinant MHCK A GST-tagged catalytic domain by glutathione affinity chromatography Dictyostelium discoideum
2.7.11.7 recombinant MHCK B GST-tagged catalytic domain by glutathione affinity chromatography Dictyostelium discoideum
2.7.11.20 recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + myosin heavy-chain
-
 Dictyostelium discoideum ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 additional information substrate specificity of the MHCK A catalytic domain: a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by MHCK A. Phosphorylation by MHCK A is enhanced 2 to 4fold by basic residues in the P+2, P+3 and P+4 positions, with a strong preference for threonine as the phosphoacceptor amino acid, overview Dictyostelium discoideum ?
-
 ?
2.7.11.7 additional information substrate specificity of the MHCK B catalytic domain: a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by MHCK B. Phosphorylation by MHCK B is further enhanced 8fold by a basic residue in the P+2 position, strong preference for threonine as the phosphoacceptor amino acid, overview Dictyostelium discoideum ?
-
 ?
2.7.11.20 ATP + [eukaryotic translation elongation factor 2] eEF2 is involved in eEF2 in neurite outgrowth regulation mechanism, since phosphorylated eEF2 inhibits mRNA translation via inhibition of eEF2-ribosome binding, calcium elevation appears to inhibit mRNA translation in growth cones by a synergistic mechanism involving regulation of EF2K, S6K, and eEF2 itself, overview Mus musculus ADP + [eukaryotic translation elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [eukaryotic translation elongation factor 2] the enzyme exhibits strong preference for threonine as the phosphoacceptor amino acid, substrate specificity and phosphoacceptor amino acid specificity, overview Mus musculus ADP + [eukaryotic translation elongation factor 2] phosphate
-
 ?
2.7.11.20 ATP + [MHCK A peptide substrate] i.e. YAYDTRYRR Mus musculus ADP + [MHCK A peptide substrate] phosphate
-
 ?
2.7.11.20 additional information a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by eEF-2K, eEF-2K requires basic residues in both the P+1 and P+3 positions to recognize peptide substrates, overview Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 MHCK A
-
 Dictyostelium discoideum
2.7.11.7 MHCK B
-
 Dictyostelium discoideum
2.7.11.7 myosin II heavy chain kinases A
-
 Dictyostelium discoideum
2.7.11.7 myosin II heavy chain kinases B
-
 Dictyostelium discoideum
2.7.11.20 EEF-2K
-
 Mus musculus
2.7.11.20 eukaryotic elongation factor-2 kinase
-
 Mus musculus
2.7.11.20 More MHCKs and eEF-2Ks comprise a distinct subgroup within the alpha kinase famiily Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 25
-
 assay at Dictyostelium discoideum
2.7.11.20 25
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7
-
 assay at Dictyostelium discoideum
2.7.11.20 7
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum
2.7.11.20 ATP
-
 Mus musculus