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Literature summary extracted from

  • Guianvarch, D.; Guangqi E, D.; Drujon, T.; Rey, C.; Wang, Q.; Ploux, O.
    Identification of inhibitors of the E. coli cyclopropane fatty acid synthase from the screening of a chemical library: In vitro and in vivo studies (2008), Biochim. Biophys. Acta, 1784, 1652-1658.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.79 recombinantly expressed in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.79 (12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene
-
 Escherichia coli
2.1.1.79 (1R,7aR)-hexahydro-1H-pyrrolizin-1-ylmethyl 4-hydroxy-3,5-dimethoxybenzoate
-
 Escherichia coli
2.1.1.79 1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole
-
 Escherichia coli
2.1.1.79 2,5,11-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole
-
 Escherichia coli
2.1.1.79 3beta,5alpha-17a-aza-D-homoandrostan-3-ol
-
 Escherichia coli
2.1.1.79 5'-S-[2-(decylamino)ethyl]-5'-thioadenosine this compound is also found to completely inhibit cyclopropanation of the phospholipids in growing Escherichia coli cells in vivo, at 0.15 mM Escherichia coli
2.1.1.79 9-methoxy-1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole
-
 Escherichia coli
2.1.1.79 dioctylamine
-
 Escherichia coli
2.1.1.79 sinefungin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.79 additional information
-
 additional information Km (phospholipids): 0.5 mg/ml. Since a mixture of phospholipids prepared from an Escherichia coli K12 strain is used, the Km constant is only an apparent constant given in mg/mL unit rather than molar unit Escherichia coli
2.1.1.79 0.08
-
 S-adenosyl-L-methionine
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.79 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.79 S-adenosyl-L-methionine + phospholipids
-
 Escherichia coli S-adenosyl-L-homocysteine + phospholipid + cyclopropane fatty acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.79 CFAS
-
 Escherichia coli
2.1.1.79 cyclopropane fatty acid synthase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.79 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.79 7.4
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.79 0.00013
-
 dioctylamine competitive inhibitor with respect to phospholipids Escherichia coli
2.1.1.79 0.00026
-
 dioctylamine uncompetitive inhibitor with respect to S-adenosyl-L-methionine Escherichia coli
2.1.1.79 0.0005
-
 sinefungin competitive inhibitor with respect to S-adenosyl-L-methionine Escherichia coli
2.1.1.79 0.0011
-
 (12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene inhibits CAFS in a mixed-type fashion with respect to phospholipids Escherichia coli
2.1.1.79 0.0014
-
 (12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene inhibits the CFAS non-competitively with respect to AdoMet Escherichia coli
2.1.1.79 0.006
-
 5'-S-[2-(decylamino)ethyl]-5'-thioadenosine apparent inhibition constant when the phospholipids are the variable substrates. Noncompetitive inhibitor with respect to both substrates Escherichia coli
2.1.1.79 0.0072
-
 sinefungin non-competitive inhibitor with respect to the phospholipids Escherichia coli
2.1.1.79 0.016
-
 5'-S-[2-(decylamino)ethyl]-5'-thioadenosine apparent inhibition constant when S-adenosyl-L-methionine is the variable substrates. Noncompetitive inhibitor with respect to both substrates Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.79 0.001
-
-
 Escherichia coli 9-methoxy-1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole
2.1.1.79 0.004
-
-
 Escherichia coli dioctylamine
2.1.1.79 0.004
-
-
 Escherichia coli 1,2,5-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole
2.1.1.79 0.005
-
-
 Escherichia coli 2,5,11-trimethyl-2,3,4,6-tetrahydro-1H-pyrido[4,3-b]carbazole
2.1.1.79 0.007
-
-
 Escherichia coli (12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene
2.1.1.79 0.009
-
-
 Escherichia coli sinefungin
2.1.1.79 0.009
-
-
 Escherichia coli 3beta,5alpha-17a-aza-D-homoandrostan-3-ol
2.1.1.79 0.01
-
-
 Escherichia coli 5'-S-[2-(decylamino)ethyl]-5'-thioadenosine
2.1.1.79 0.01
-
-
 Escherichia coli (1R,7aR)-hexahydro-1H-pyrrolizin-1-ylmethyl 4-hydroxy-3,5-dimethoxybenzoate