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Literature summary extracted from

  • Lee, S.; Belyaeva, O.V.; Kedishvili, N.Y.
    Effect of lipid peroxidation products on the activity of human retinol dehydrogenase 12 (RDH12) and retinoid metabolism (2008), Biochim. Biophys. Acta, 1782, 421-425.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.300 medicine transfection with retinol dehydrogenase 12 protects cells against nonanal-induced toxicity but is ineffective against 4-hydroxynonenal. 4-Hydroxynonenal strongly inhibits the activities of lecithin:retinol acyl transferase and aldehyde dehydrogenase, resulting in decreased levels of retinyl esters and retinoic acid and accumulation of unesterified retinol Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.300 expression in HEK-293 cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.300 I51N site-directed mutagenesis, significant activity in vitro. Dramatically reduced affinity for NADPH results in loss of function within cells Homo sapiens
1.1.1.300 L99I site-directed mutagenesis, about 30% of wild-type activity Homo sapiens
1.1.1.300 additional information transfection with retinol dehydrogenase 12 protects cells against nonanal-induced toxicity but is ineffective against 4-hydroxynonenal Homo sapiens
1.1.1.300 S175P site-directed mutagenesis, no catalytic activity. Protein is stable and abundantly expressed Homo sapiens
1.1.1.300 T49M site-directed mutagenesis, significant activity in vitro. Dramatically reduced affinity for NADPH results in loss of function within cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.300 Nonanal inhibits the activity towards retinaldehyde Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.300 0.00006
-
all-trans-retinal wild-type Homo sapiens
1.1.1.300 0.00028
-
all-trans-retinal mutant T49M Homo sapiens
1.1.1.300 0.00041
-
all-trans-retinal mutant I51N Homo sapiens
1.1.1.300 0.0007
-
NADPH wild-type Homo sapiens
1.1.1.300 0.0032
-
NADP+ wild-type Homo sapiens
1.1.1.300 0.033
-
NADPH mutant T49M Homo sapiens
1.1.1.300 0.1
-
NADP+ mutant T49M Homo sapiens
1.1.1.300 0.105
-
NADPH mutant I51N Homo sapiens
1.1.1.300 0.35
-
NADP+ mutant I51N Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+ Homo sapiens
-
all-trans-retinol + NADP+
-
?
1.1.1.300 additional information Homo sapiens although bi-directional in vitro, in living cells, RDH12 acts exclusively as a retinaldehyde reductase, shifting the retinoid homeostasis toward the increased levels of retinol and decreased levels of bioactive retinoic acid. The retinaldehyde reductase activity of RDH12 protects the cells from retinaldehyde-induced cell death ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.300 Homo sapiens
-
isoform RDH12
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.300 all-trans-retinal + NADPH + H+
-
Homo sapiens all-trans-retinol + NADP+
-
?
1.1.1.300 additional information although bi-directional in vitro, in living cells, RDH12 acts exclusively as a retinaldehyde reductase, shifting the retinoid homeostasis toward the increased levels of retinol and decreased levels of bioactive retinoic acid. The retinaldehyde reductase activity of RDH12 protects the cells from retinaldehyde-induced cell death Homo sapiens ?
-
?
1.1.1.300 retinal + NADH
-
Homo sapiens retinol + NAD+
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.300 0.18
-
all-trans-retinal wild-type Homo sapiens
1.1.1.300 0.21
-
all-trans-retinal mutant I51N Homo sapiens
1.1.1.300 0.4
-
all-trans-retinal mutant T49M Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.300 NADP+
-
Homo sapiens
1.1.1.300 NADPH
-
Homo sapiens